Proteasome inhibitors induce changes in chromatin structure characteristic of senescent human fibroblasts

被引：11

作者：

Ukekawa, R

Maegawa, N

Mizutani, E

Fujii, M

Ayusawa, D

机构：

[1] Yokohama City Univ, Kihara Inst Biol Res, Yokohama, Kanagawa 2440813, Japan

[2] Yokohama City Univ, Grad Sch Integrated Sci, Yokohama, Kanagawa 2440813, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 11期

关键词：

cellular senescence; proteasome; heterochromatin; inhibitor;

D O I：

10.1271/bbb.68.2395

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhibitors of proteasome induced premature senescence in normal human fibroblasts. Besides morphological alteration and expression of senescence marker genes, these cells manifested senescence-associated heterochromatic foci under staining of the nuclei with DAPI similar to normally senescent cells. These results suggest that declining ability in protein degradation may be involved in the formation of heterochromatic foci in senescent fibroblasts.

引用

收藏

页码：2395 / 2397

页数：3

相关论文

共 9 条

[1] Deconstructing PML-induced premature senescence [J].

Bischof, O ;

Kirsh, O ;

Pearson, M ;

Itahana, K ;

Pelicci, PG ;

Dejean, A .

EMBO JOURNAL, 2002, 21 (13) :3358-3369

[2] Central role of the proteasome in senescence and survival of human fibroblasts - Induction of a senescence-like phenotype upon its inhibition and resistance to stress upon its activation [J].

Chondrogianni, N ;

Stratford, FLL ;

Trougakos, IP ;

Friguet, B ;

Rivett, AJ ;

Gonos, ES .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (30) :28026-28037

[3] Proteasome-dependent dispersal of PML nuclear bodies in response to alkylating DNA damage [J].

Conlan, LA ;

McNees, CJ ;

Heierhorst, J .

ONCOGENE, 2004, 23 (01) :307-310

[4] A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO [J].

DIMRI, GP ;

LEE, XH ;

BASILE, G ;

ACOSTA, M ;

SCOTT, C ;

ROSKELLEY, C ;

MEDRANO, EE ;

LINSKENS, M ;

RUBELJ, I ;

PEREIRASMITH, O ;

PEACOCKE, M ;

CAMPISI, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9363-9367

[5] LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS [J].

HAYFLICK, L .

EXPERIMENTAL CELL RESEARCH, 1965, 37 (03) :614-&

[6] DNA topoisomerase inhibitors induce reversible senescence in normal human fibroblasts [J].

Michishita, E ;

Nakabayashi, K ;

Ogino, H ;

Suzuki, T ;

Fujii, M ;

Ayusawa, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 253 (03) :667-671

[7]

Michishita E, 1999, J BIOCHEM-TOKYO, V126, P1052

[8] Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence [J].

Narita, M ;

Nunez, S ;

Heard, E ;

Narita, M ;

Lin, AW ;

Hearn, SA ;

Spector, DL ;

Hannon, GJ ;

Lowe, SW .

CELL, 2003, 113 (06) :703-716

[9] PML regulates p53 acetylation and premature senescence induced by oncogenic Ras [J].

Pearson, M ;

Carbone, R ;

Sebastiani, C ;

Cioce, M ;

Fagioli, M ;

Saito, S ;

Higashimoto, Y ;

Appella, E ;

Minucci, S ;

Pandolfi, PP ;

Pelicci, PG .

NATURE, 2000, 406 (6792) :207-210