T cell dependent B cell activation occurs during the induction of T cell anergy by staphylococcal enterotoxin B in mice.

被引:4
作者
Abdul-Majid, KB [1 ]
Abedi-Valurgerdi, M [1 ]
机构
[1] Univ Stockholm, Arrhenius Labs Nat Sci, Dept Immunol, S-10691 Stockholm, Sweden
关键词
D O I
10.3109/08820139809070891
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Staphylococcal enterotoxin B (SEB) can activate specific T cell clones bearing specific TcR V beta domains together with MHC class II ligands on accessory cells. The release of proinflammatory cytokines is the consequence of this activation as well as the main pathological aspect involved in SEB infection. This current study looked at the active role of both T and B cells during the induction of anergy by SEB in vivo. Euthymic and nude BALB/c mice were injected with SEB and over a period of 8 days, cells from the spleen and sera from the blood were collected. After a single injection with SEB (50 mu g/mouse), a transient increase of CD4(+)V beta 8(+) T cells were detected after 2 days followed by a decrease after 4 days, which persisted until day 8. These clones were rendered anergic upon restimulation in vitro with SEB. Interestingly, cells taken out 2 days after SEB injection, exhibited reduced proliferation in response to Con A. However, this response gradually recovered on days 4, 6 and 8. Furthermore, early IgM antibody production (day 2) was observed after SEB injection. SEB-induced IgM antibody production in euthymic BALB/c was found to have specificity against SEB, cardiolipin (CL) and phosphatidylethanolamine (PE). SEB-treated nude mice did not produce antibody secreting cells in response to SEB, indicating that this process is T cell dependent.
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页码:73 / 88
页数:16
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