Determination of thiodicarb using a biosensor based on alfalfa sprout peroxidase immobilized in self-assembled monolayers

被引：30

作者：

Moccelini, Sally K. ^{[1
]}

Vieira, Iolanda C. ^{[1
]}

de Lima, Fabio ^{[2
]}

Lucca, Bruno G. ^{[2
]}

Barbosa, Antonio M. J. ^{[2
]}

Ferreira, Valdir S. ^{[2
]}

机构：

[1] Univ Fed Santa Catarina, Dept Quim, Lab Biossensores, BR-88040970 Florianopolis, SC, Brazil

[2] Univ Fed Mato Grosso do Sul, Dept Quim, BR-79070900 Campo Grande, MS, Brazil

来源：

TALANTA | 2010年 / 82卷 / 01期

关键词：

Biosensor; Thiodicarb; Alfalfa sprouts; Self-assembled monolayers; L-CYSTEINE DETERMINATION; CARBON-PASTE ELECTRODE; HORSERADISH-PEROXIDASE; AMPEROMETRIC BIOSENSOR; CARBAMATE PESTICIDES; INHIBITION BIOSENSOR; CRUDE EXTRACT; ENZYME; WATER; PERFORMANCE;

D O I：

10.1016/j.talanta.2010.04.015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor based on alfalfa sprout (Medicago sativa) homogenate as a source of peroxidase is proposed for the determination of thiodicarb by square-wave voltammetry. This enzyme was immobilized in self-assembled monolayers of L-cysteine on a gold electrode. Several parameters were investigated to evaluate the optimum conditions for operation of the biosensor. The analytical curve was linear for thiodicarb concentrations of 2.27 x 10(-6) to 4.40 x 10(-5) mol L-1 with a detection limit of 5.75 x 10(-7) mol L-1. The lifetime of the Au-alfalfa sprout-SAMs was 20 days (at least 220 determinations). The average recovery of thiodicarb from samples of vegetable extracts ranged from 99.02 to 101.04%. The results obtained for thiodicarb in vegetable extracts using the proposed method are in close agreement with those using a high performance liquid chromatography procedure at the 95% confidence level. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：164 / 170

页数：7

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