Monomeric γ-tubulin nucleates microtubules

被引：54

作者：

Leguy, R ^{[1
]}

Melki, R ^{[1
]}

Pantaloni, D ^{[1
]}

Carlier, MF ^{[1
]}

机构：

[1] CNRS, Lab Enzymol & Biochim Struct, F-91198 Gif Sur Yvette, France

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 29期

关键词：

D O I：

10.1074/jbc.M000688200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

gamma-Tubulin is required for nucleation and polarized organization of microtubules in vivo. The mechanism of microtubule nucleation by gamma-tubulin and the role of associated proteins is not understood. Here we show that in vitro translated monomeric gamma-tubulin nucleates microtubules by lowering the size of the nucleus from seven to three tubulin subunits, In capping the minus end with high affinity (10(10) M-1) and a binding stoichiometry of one molecule of gamma-tubulin/microtubule, gamma-tubulin establishes the critical concentration of the plus end in the medium and prevents minus end growth. gamma-Tubulin interacts strongly with beta-tubulin. A structural model accounts for these results.

引用

页码：21975 / 21980

页数：6

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