QUANTITATIVE LOCALIZATION OF CHROMOSOMAL LOCI BY IMMUNOFLUORESCENCE

被引：17

作者：

Brickner, Donna Garvey ^{[1
]}

Light, William ^{[1
]}

Brickner, Jason H. ^{[1
]}

机构：

[1] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA

来源：

METHODS IN ENZYMOLOGY, VOL 470: GUIDE TO YEAST GENETICS:: FUNCTIONAL GENOMICS, PROTEOMICS, AND OTHER SYSTEMS ANALYSIS, 2ND EDITION | 2010年 / 470卷

关键词：

NUCLEAR PERIPHERY; GENES; ORGANIZATION; REVEALS; WELL; DNA;

D O I：

10.1016/S0076-6879(10)70022-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA within the yeast nucleus is spatially organized. Yeast telomeres cluster together at the nuclear periphery, centromeres cluster together near the spindle pole body, and both the rDNA repeats and tRNA genes cluster within the nucleolus. Furthermore, the localization of individual genes to subnuclear compartments can change with changes in transcriptional status. As such, yeast researchers interested in understanding nuclear events may need to determine the subnuclear localization of parts of the genome. This chapter describes a straightforward quantitative approach using immunofluorescence and confocal microscopy to localize chromosomal loci with respect to well characterized nuclear landmarks.

引用

页码：569 / 580

页数：12

共 29 条

[1] 3′-end formation signals modulate the association of genes with the nuclear periphery as well as mRNP dot formation [J].

Abruzzi, Katharine C. ;

Belostotsky, Dmitry A. ;

Chekanova, Julia A. ;

Dower, Ken ;

Rosbash, Michael .

EMBO JOURNAL, 2006, 25 (18) :4253-4262

[2] High-resolution statistical mapping reveals gene territories in live yeast [J].

Berger, Axel B. ;

Cabal, Ghislain G. ;

Fabre, Emmanuelle ;

Duong, Tarn ;

Buc, Henri ;

Nehrbass, Ulf ;

Olivo-Marin, Jean-Christophe ;

Gadal, Olivier ;

Zimmer, Christophe .

NATURE METHODS, 2008, 5 (12) :1031-1037

[3]

Born M, 1998, PRINCIPLES OPTICS

[4] H2A.z-mediated localization of genes at the nuclear periphery confers epigenetic memory of previous transcriptional state [J].

Brickner, Donna Garvey ;

Cajigas, Ivelisse ;

Fondufe-Mittendorf, Yvonne ;

Ahmed, Sara ;

Lee, Pei-Chih ;

Widom, Jonathan ;

Brickner, Jason H. .

PLOS BIOLOGY, 2007, 5 (04) :704-716

[5] Gene recruitment of the activated INO1 locus to the nuclear membrane [J].

Brickner, JH ;

Walter, P .

PLOS BIOLOGY, 2004, 2 (11) :1843-1853

[6] SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope [J].

Cabal, Ghislain G. ;

Genovesio, Auguste ;

Rodriguez-Navarro, Susana ;

Zimmer, Christophe ;

Gadal, Olivier ;

Lesne, Annick ;

Buc, Henri ;

Feuerbach-Fournier, Frank ;

Olivo-Marin, Jean-Christophe ;

Hurt, Eduard C. ;

Nehrbass, Ulf .

NATURE, 2006, 441 (7094) :770-773

[7] Developmentally induced changes in transcriptional program alter spatial organization across chromosomes [J].

Casolari, JM ;

Brown, CR ;

Drubin, DA ;

Rando, OJ ;

Silver, PA .

GENES & DEVELOPMENT, 2005, 19 (10) :1188-1198

[8] Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization [J].

Casolari, JM ;

Brown, CR ;

Komili, S ;

West, J ;

Hieronymus, H ;

Silver, PA .

CELL, 2004, 117 (04) :427-439

[9] Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP [J].

Chekanova, Julia A. ;

Abruzzi, Katharine C. ;

Rosbash, Michael ;

Belostotsky, Dmitry A. .

RNA, 2008, 14 (01) :66-77

[10] Long-range directional movement of an interphase chromosome site [J].

Chuang, CH ;

Carpenter, AE ;

Fuchsova, B ;

Johnson, T ;

de Lanerolle, P ;

Belmont, AS .

CURRENT BIOLOGY, 2006, 16 (08) :825-831

← 1 2 3 →