Imaging of spheres and surface profiling by confocal microscopy

被引:34
作者
Aguilar, JF
Lera, M
Sheppard, CJR
机构
[1] Univ Sydney, Sch Phys A 28, Dept Phys Opt, Sydney, NSW 2006, Australia
[2] Univ Cagliari, Dipartimento Ingn Meccan, I-09123 Cagliari, Italy
[3] Univ Sydney, Australian Key Ctr Microscopy & Microanal, Sydney, NSW 2006, Australia
关键词
D O I
10.1364/AO.39.004621
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
An investigation of the imaging of spheres can aid in understanding details of the surface profiling method of confocal microscopy. The use of semicircular masks to eliminate artifacts in confocal profiling is investigated experimentally. A theoretical treatment of image formation for a spherical object in reflection confocal microscopy is presented. For large spheres a simple approximate theory is described and is shown to be equivalent to application of the Kirchhoff theory for surface scattering; (C) 2000 Optical Society of America OCIS codes: 180.0180, 180.1790, 170.6900, 120.6660.
引用
收藏
页码:4621 / 4628
页数:8
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