Histone H1 enhances synergistic activation of the MMTV promoter in chromatin

被引:63
作者
Koop, R
Di Croce, L
Beato, M
机构
[1] Univ Marburg, IMT, D-35033 Marburg, Germany
[2] Univ Pompeu Fabra, CRG, E-08003 Barcelona, Spain
关键词
H1; phosphorylation; linker histone; nucleosome structure; progesterone receptor; transcriptional synergism;
D O I
10.1093/emboj/cdg052
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Minichromosomes assembled on the mouse mammary tumor virus (MMTV) promoter in vitro exhibit positioned nucleosomes, one of which covers the binding sites for progesterone receptor (PR) and nuclear factor 1 (NF1). Incorporation of histone H1 into MMTV minichromosomes improves the stability of this nucleosome and decreases basal transcription from the MMTV promoter, as well as its response to either PR or NF1. However, histone HI-containing minichromosomes display better PR binding and support a more efficient synergism between PR and NF1, leading to enhanced transcription initiation. A mutant MMTV promoter lacking positioned nucleosomes does not display enhanced transcriptional synergism in the presence of H1. Binding of PR leads to phosphorylation of H1, which leaves the promoter upon transcription initiation. Thus, H1 assumes a complex and dynamic role in the regulation of the MMTV promoter.
引用
收藏
页码:588 / 599
页数:12
相关论文
共 58 条
[1]   Hormone-mediated dephosphorylation of specific histone H1 isoforms [J].
Banks, GC ;
Deterding, LJ ;
Tomer, KB ;
Archer, TK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (39) :36467-36473
[2]   Histone H1 phosphorylation by cdk2 selectively modulates mouse mammary tumor virus transcription through chromatin remodeling [J].
Bhattacharjee, RN ;
Banks, GC ;
Trotter, KW ;
Lee, HL ;
Archer, TK .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (16) :5417-5425
[3]  
Bonte E, 1999, METH MOL B, V119, P187
[4]   SPECIFIC REGULATION OF XENOPUS CHROMOSOMAL 5S RIBOSOMAL-RNA GENE-TRANSCRIPTION IN-VIVO BY HISTONE H1 [J].
BOUVET, P ;
DIMITROV, S ;
WOLFFE, AP .
GENES & DEVELOPMENT, 1994, 8 (10) :1147-1159
[5]   THE TRANSCRIPTIONALLY-ACTIVE MMTV PROMOTER IS DEPLETED OF HISTONE H1 [J].
BRESNICK, EH ;
BUSTIN, M ;
MARSAUD, V ;
RICHARDFOY, H ;
HAGER, GL .
NUCLEIC ACIDS RESEARCH, 1992, 20 (02) :273-278
[6]   A POTENT GAL4 DERIVATIVE ACTIVATES TRANSCRIPTION AT A DISTANCE INVITRO [J].
CAREY, M ;
LEATHERWOOD, J ;
PTASHNE, M .
SCIENCE, 1990, 247 (4943) :710-712
[7]   A MECHANISM FOR SYNERGISTIC ACTIVATION OF A MAMMALIAN GENE BY GAL4 DERIVATIVES [J].
CAREY, M ;
LIN, YS ;
GREEN, MR ;
PTASHNE, M .
NATURE, 1990, 345 (6273) :361-364
[8]  
CHAVEZ S, 1995, MOL CELL BIOL, V15, P6987
[9]   Nucleosome-mediated synergism between transcription factors on the mouse mammary tumor virus promoter [J].
Chavez, S ;
Beato, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (07) :2885-2890
[10]   Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase [J].
Chen, HW ;
Lin, RJ ;
Xie, W ;
Wilpitz, D ;
Evans, RM .
CELL, 1999, 98 (05) :675-686