Localization of the Leptospira interrogans metF gene on the CII secondary chromosome

被引：6

作者：

Bourhy, P ^{[1
]}

Saint Girons, I ^{[1
]}

机构：

[1] Inst Pasteur, Unite Bacteriol Mol & Med, F-75724 Paris 15, France

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 191卷 / 02期

关键词：

Leptospira interrogans; metF; methylene tetrahydrofolate reductase; thermolabile enzyme; secondary chromosome;

D O I：

10.1016/S0378-1097(00)00393-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An open reading frame of 885 nucleotides was identified as the Leptospira interrogans metF gene. The deduced amino acid sequence (294 amino acids) showed similarities with Escherichia coli methylene tetrahydrofolate reductase (MetF or MTHFR) (33% identity) and with the N-terminal part of human MTHFR (33% identity). The L. interrogans metF gene complements an E. coli metF mutant to prototrophy, suggesting the functionality of the folate branch converging to form methionine. In addition, the L. interrogans MetF was Found to be thermolabile. The metF gene belonged to the CII secondary chromosome, in contrast to the previously isolated metY and metX genes, which have been localized to the: CI chromosome of Leptospira sp. (C) 2000 Federation of European Microbiological Societies. published by Elsevier Science B.V. All rights reserved.

引用

页码：259 / 263

页数：5

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