Regulation of Rubisco activation in antisense plants of tobacco containing reduced levels of Rubisco activase

被引:65
作者
Hammond, ET
Andrews, TJ
Mott, KA
Woodrow, IE [1 ]
机构
[1] Univ Melbourne, Sch Bot, Parkville, Vic 3052, Australia
[2] Australian Natl Univ, Sch Biol Sci, Canberra, ACT 2601, Australia
[3] Utah State Univ, Dept Biol, Logan, UT 84322 USA
关键词
D O I
10.1046/j.1365-313X.1998.00103.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Following an increase in photon flux density (PFD), ribulose bisphosphate carboxylase/oxygenase (Rubisco) undergoes a slow activation which substantially limits the rate of photosynthesis. This activation process is mediated in part by Rubisco activase. Antisense DNA plants of tobacco were used to quantify the degree to which activase limits Rubisco activation. Reductions in leaf activase content caused proportional reductions in the rate of Rubisco activation following a PFD increase from 110 to 1200 mu mol m(-2) sec(-1). This was the case for activase levels up to and slightly beyond normal wild-type activase levels. Activase therefore has a flux control coefficient of unity with respect to the Rubisco activation flux. Such a high control coefficient has rarely been measured for any metabolic system, and this is the highest control coefficient measured for an important photosynthetic flux. In contrast, the rate of Rubisco inactivation in leaves following a drop in PFD of 1200 to 110 mu mol m(-2) sec(-1) was unchanged by a 60% reduction in activase levels. Despite the high degree of control that activase exerts over the rate of activation, and thus non-steady-state photosynthesis, it was shown that steady-state photosynthesis was largely unaffected by activase concentration until it was reduced below approximately 15% of the wild-type level. The significance of these results and their implications for published models of Rubisco activation are discussed.
引用
收藏
页码:101 / 110
页数:10
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