Bio-imprinting of lipases with fatty acids

被引:53
作者
Fishman, A
Cogan, U [1 ]
机构
[1] Technion Israel Inst Technol, Dept Food Engn & Biotechnol, IL-32000 Haifa, Israel
[2] IMI TAMI Inst R&D, IL-26111 Haifa, Israel
关键词
lipases; organic solvents; bio-imprinting; biocatalysis; immobilization;
D O I
10.1016/S1381-1177(03)00032-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bio-imprinting of lipases with fatty acids was shown to be a feasible, effective method for obtaining highly active enzymes in organic solvents. The increase inactivity was dependent on the enzyme type, the solvent type and the imprint molecule itself. A correlation between the initial activity of caprylic acid-imprinted Candida rugosa lipase (CRL), and solvent hydrophobicity was observed. In addition, the combination of bio-imprinting with adsorption onto an inert support such as celite, proved to be a powerful technique for obtaining an even more active and stable enzyme preparations. In the case of lipase from Pseudomonas sp., the increase in activity resulting from bio-imprinting with caprylic acid and immobilization onto celite, was 20-fold. Porcine pancreatic lipase (PPL), treated in the same manner, retained 70% of its initial activity at the end of 20 consecutive reaction cycles, compared to only 20% residual activity for the non-treated control. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:193 / 202
页数:10
相关论文
共 33 条
[1]   SOLVENT DIELECTRIC EFFECTS ON PROTEIN DYNAMICS [J].
AFFLECK, R ;
HAYNES, CA ;
CLARK, DS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :5167-5170
[2]  
ALDERCREUTZ P, 1996, ENZYMATIC REACTIONS, P9
[3]  
Carrea G, 2000, ANGEW CHEM INT EDIT, V39, P2226
[4]  
FABER K, 2000, BIOTRANSFORMATIONS O, P400
[5]   Fatty-acid-modified enzymes as enantioselective catalysts in microaqueous organic media [J].
Fishman, A ;
Basheer, S ;
Shatzmiller, S ;
Cogan, U .
BIOTECHNOLOGY LETTERS, 1998, 20 (06) :535-538
[6]   Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues [J].
Furukawa, S ;
Ono, T ;
Ijima, H ;
Kawakami, K .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 15 (1-3) :65-70
[7]   Improving lipase activity in solvent-free media by interfacial activation-based molecular bioimprinting [J].
GonzalezNavarro, H ;
Braco, L .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1997, 3 (1-4) :111-119
[8]   RELATIONSHIP BETWEEN SURFACE HYDROPHILICITY OF A PROTEIN AND ITS STABILITY AGAINST DENATURATION BY ORGANIC-SOLVENTS [J].
KHMELNITSKY, YL ;
BELOVA, AB ;
LEVASHOV, AV ;
MOZHAEV, VV .
FEBS LETTERS, 1991, 284 (02) :267-269
[9]   Improving enzymes by using them in organic solvents [J].
Klibanov, AM .
NATURE, 2001, 409 (6817) :241-246
[10]   Improvement of enantioselectivity by immobilized imprinting of epoxide hydrolase from Rhodotorula glutinis [J].
Kronenburg, NAE ;
de Bont, JAM ;
Fischer, L .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 16 (3-4) :121-129