Saccharomyces cerevisiae Coq9 polypeptide is a subunit of the mitochondrial coenzyme Q biosynthetic complex

被引：70

作者：

Hsieh, Edward J.

Gin, Peter

Gulmezian, Melissa

Tran, UyenPhuong C.

Saiki, Ryoichi

Marbois, Beth N.

Clarke, Catherine F. ^{[1
]}

机构：

[1] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Div Cardiol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Inst Mol Biol, Dept Chem & Biochem, Los Angeles, CA 90095 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2007年 / 463卷 / 01期

关键词：

coenzyme Q; mitochondrial protein; multi-subunit polypeptide biosynthetic complex; Saccharomyces cerevisiae; ubiquinone; yeast;

D O I：

10.1016/j.abb.2007.02.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coenzyme Q (Q is a redox active lipid that is an essential component of the electron transport chain. Here, we show that steady state levels of Coq3, Coq4, Coq6, Coq7 and Coq9 polypeptides in yeast mitochondria are dependent on the expression of each of the other COQ genes. Submitochondrial localization studies indicate Coq9p is a peripheral membrane protein on the matrix side of the mitochondrial inner membrane. To investigate whether Coq9p is a component of a complex of Q-biosynthetic proteins, the native molecular mass of Coq9p was determined by Blue Native-PAGE. Coq9p was found to co-migrate with Coq3p and Coq4p at a molecular mass of approximately 1 MDa. A direct physical interaction was shown by the immunoprecipitation of HA-tagged Coq9 polypeptide with Coq4p, Coq5p, Coq6p and Coq7p. These findings, together with other work identifying Coq3p and Coq4p interactions, identify at least six Coq polypeptides in a multi-subunit Q biosynthetic complex. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：19 / 26

页数：8

共 42 条

[1] Yeast mitochondrial F1F0-ATP synthase exists as a dimer:: identification of three dimer-specific subunits [J].