Circulating tumor cells with a putative stem cell phenotype in peripheral blood of patients with breast cancer

被引：218

作者：

Theodoropoulos, Panayiotis A. ^{[1
]}

Polioudaki, Hara ^{[1
]}

Agelaki, Sofia ^{[2
,3
]}

Kallergi, Galatea ^{[2
]}

Saridaki, Zacharenia ^{[2
,3
]}

Mavroudis, Dimitris ^{[2
,3
]}

Georgoulias, Vassilis ^{[2
,3
]}

机构：

[1] Univ Crete, Sch Med, Dept Biochem, Iraklion 71003, Greece

[2] Univ Crete, Sch Med, Tumor Cell Biol Lab, Iraklion 71003, Greece

[3] Univ Gen Hosp Heraklion, Dept Med Oncol, Iraklion, Greece

来源：

CANCER LETTERS | 2010年 / 288卷 / 01期

关键词：

Micrometastasis; Circulating tumor cells; Breast cancer; Stem cells; Triple-marker immunofluorescence; BONE-MARROW; IDENTIFICATION; CD44(+)/CD24(-/LOW); DISEASE;

D O I：

10.1016/j.canlet.2009.06.027

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The CD44(+)/CD24(-/low) and ALDH1(+) cell phenotypes are associated with sternness and enhanced tumorigenic potential in breast cancer. We assessed the expression of CD44, CD24 and ALDH1 on tumor cells circulating in the peripheral blood (CTCs) of patients with metastatic breast cancer using triple-marker immurrofluorescence microscopy. Among a total of 1439 CTCs identified in 20 (66.7%) out of 30 patients, 35.2% had the stem-like/tumorigenic phenotype CD44(+)/CD24(-/low), whereas 17.7% of the CTCs analyzed in seven patients, were ALDH1(high)/CD24(-/low). In conclusion, we report the existence of a subpopulation of CTCs with putative stem cell progenitor phenotypes in patients with metastatic breast cancer. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：99 / 106

页数：8

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