Distinct trafficking and localization of STEVOR proteins in three stages of the Plasmodium falciparum life cycle

被引:73
作者
McRobert, L
Preiser, P
Sharp, S
Jarra, W
Kaviratne, M
Taylor, MC
Renia, L
Sutherland, CJ
机构
[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Med, London WC1E 7HT, England
[2] Natl Inst Med Res, Div Parasitol, London NW7 1AA, England
[3] Nanyang Technol Univ, Sch Biol Sci, Singapore 2263, Singapore
[4] Inst Cochin Genet Mol, INSERM, U567, CNRS UMR 8104,Dept Immunol, F-75014 Paris, France
关键词
D O I
10.1128/IAI.72.11.6597-6602.2004
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The genome of Plasmodium falciparum harbors three extensive multigene families, var, rif, and stevor (for subtelomeric variable open reading frame), located mainly in the subtelomeric regions of the parasite's 14 chromosomes. STEVOR variants are known to be expressed in asexual parasites, but no function has as yet been ascribed to this protein family. We have examined the expression of STEVOR proteins in intraerythrocytic sexual stages, gametocytes, and extracellular sporozoites isolated from infected Anopheles mosquitoes. In gametocytes, stevor transcripts appear transiently early in development but STEVOR proteins persist for several days and are transported out of the parasite, travel through the host cell cytoplasm, and localize to the erythrocyte plasma membrane. In contrast to asexual parasites, gametocytes move STEVOR to the periphery via a trafficking pathway independent of Maurer's clefts. In sporozoites, STEVOR appear dispersed throughout the cytoplasm in vesicle-like structures. The pattern of STEVOR localization we have observed in gametocytes and sporozoites differs significantly from that in asexual parasite stages. STEVOR variants are therefore likely to perform different functions in each stage of the parasites life cycle in which they occur.
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页码:6597 / 6602
页数:6
相关论文
共 28 条
[1]   High-throughput sequence typing of T-cell epitope polymorphisms in Plasmodium falciparum circumsporozoite protein [J].
Alloueche, A ;
Silveira, H ;
Conway, DJ ;
Bojang, K ;
Doherty, T ;
Cohen, J ;
Pinder, M ;
Greenwood, BM .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 106 (02) :273-282
[2]   SUBCELLULAR-LOCALIZATION OF PFS16, A PLASMODIUM-FALCIPARUM GAMETOCYTE ANTIGEN [J].
BAKER, DA ;
DARAMOLA, O ;
MCCROSSAN, MV ;
HARMER, J ;
TARGETT, GAT .
PARASITOLOGY, 1994, 108 :129-137
[3]   Pfsbp 1, a Maurer's cleft Plasmodium falciparum protein, is associated with the erythrocyte skeleton [J].
Blisnick, T ;
Eugenia, M ;
Betoulle, M ;
Barale, JC ;
Uzureau, P ;
Berry, L ;
Desroses, S ;
Fujioka, H ;
Mattei, D ;
Breton, CB .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 111 (01) :107-121
[4]   Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray -: art. no. R9 [J].
Bozdech, Z ;
Zhu, JC ;
Joachimiak, MP ;
Cohen, FE ;
Pulliam, B ;
DeRisi, JL .
GENOME BIOLOGY, 2003, 4 (02)
[5]   CELLULAR LOCATION AND TEMPORAL EXPRESSION OF THE PLASMODIUM-FALCIPARUM SEXUAL STAGE ANTIGEN PFS16 [J].
BRUCE, MC ;
CARTER, RN ;
NAKAMURA, K ;
AIKAWA, M ;
CARTER, R .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 65 (01) :11-22
[6]  
CARTER R, 1988, MALARIA PRINCIPLES P, P253
[7]   stevor and rif are Plasmodium falciparum multicopy gene families which potentially encode variant antigens [J].
Cheng, Q ;
Cloonan, N ;
Fischer, K ;
Thompson, J ;
Waine, G ;
Lanzer, M ;
Saul, A .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1998, 97 (1-2) :161-176
[8]   CD36-dependent adhesion and knob expression of the transmission stages of Plasmodium falciparum is stage-specific [J].
Day, KP ;
Hayward, RE ;
Smith, D ;
Culvenor, JG .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1998, 93 (02) :167-177
[9]   CHARACTERIZATION OF MURINE MONOCLONAL-ANTIBODIES AGAINST A REPETITIVE SYNTHETIC PEPTIDE FROM THE CIRCUMSPOROZOITE PROTEIN OF THE HUMAN MALARIA PARASITE, PLASMODIUM-FALCIPARUM [J].
DELGIUDICE, G ;
TOUGNE, C ;
RENIA, L ;
PONNUDURAI, T ;
CORRADIN, G ;
PESSI, A ;
VERDINI, AS ;
LOUIS, JA ;
MAZIER, D ;
LAMBERT, PH .
MOLECULAR IMMUNOLOGY, 1991, 28 (09) :1003-1009
[10]   Targeting and sequestration of truncated Pfs230 in an intraerythrocytic compartment during Plasmodium falciparum gametocytogenesis [J].
Eksi, S ;
Stump, A ;
Fanning, SL ;
Shenouda, MI ;
Fujioka, H ;
Williamson, KC .
MOLECULAR MICROBIOLOGY, 2002, 44 (06) :1507-1516