Cloning of a novel human ELF-1-related ETS transcription factor, ELFR, its characterization and chromosomal assignment relative to ELF-1

被引：11

作者：

Aryee, DNT ^{[1
]}

Petermann, R ^{[1
]}

Kos, K ^{[1
]}

Henn, T ^{[1
]}

Haas, OA ^{[1
]}

Kovar, H ^{[1
]}

机构：

[1] St Anna Kinderspital, CCRI, A-1090 Vienna, Austria

来源：

GENE | 1998年 / 210卷 / 01期

基金：

奥地利科学基金会;

关键词：

chromosome; 13q13; chromosome Xq26; Ewing tumor; EWS-Fli1;

D O I：

10.1016/S0378-1119(98)00022-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The ETS gene family encodes a group of proteins that function as transcription factors under physiological conditions and, if aberrantly expressed, can lead to cellular transformation. ETS transcription factors are characterized by a unique conserved DNA binding domain. A subset of these proteins is rearranged with EWS in Ewing tumors (ET). We recently described a spectrum of ETS genes coexpressed with EWS-FLII in an ET cell line to define proteins that potentially compete in target site selection. We now report on the cloning and characterization of a novel ETS family member, ELFR, displaying 92% homology to ELF-1 in its DNA binding domain while diverging in the rest of the protein. ELFR expression was found in a very tissue restricted pattern with the highest abundancy in placenta. We also report the chromosomal assignment of ELFR and ELF-1 to Xq26 and 13q13, respectively, by means of fluorescence in-situ hybridization (FISH). (C) 1998 Elsevier Science B.V.

引用

收藏

页码：71 / 78

页数：8

相关论文

共 27 条

[1] SUPPRESSION OF HUMAN COLORECTAL-CARCINOMA CELL-GROWTH BY WILD-TYPE-P53 [J].

BAKER, SJ ;

MARKOWITZ, S ;

FEARON, ER ;

WILLSON, JKV ;

VOGELSTEIN, B .

SCIENCE, 1990, 249 (4971) :912-915

[2] ERYTHROLEUKEMIA INDUCTION BY FRIEND MURINE LEUKEMIA-VIRUS - INSERTIONAL ACTIVATION OF A NEW MEMBER OF THE ETS GENE FAMILY, FLI-1, CLOSELY LINKED TO C-ETS-1 [J].

BENDAVID, Y ;

GIDDENS, EB ;

LETWIN, K ;

BERNSTEIN, A .

GENES & DEVELOPMENT, 1991, 5 (06) :908-918

[3] A SINGLE AMINO-ACID SUBSTITUTION IN THE ETS DOMAIN ALTERS CORE DNA-BINDING SPECIFICITY OF ETS1 TO THAT OF THE RELATED TRANSCRIPTION FACTORS ELF1 AND E74 [J].

BOSSELUT, R ;

LEVIN, J ;

ADJADJ, E ;

GHYSDAEL, J .

NUCLEIC ACIDS RESEARCH, 1993, 21 (22) :5184-5191

[4] SPECIFICITIES OF PROTEIN PROTEIN AND PROTEIN DNA INTERACTION OF GABP-ALPHA AND 2 NEWLY DEFINED ETS-RELATED PROTEINS [J].

BROWN, TA ;

MCKNIGHT, SL .

GENES & DEVELOPMENT, 1992, 6 (12B) :2502-2512

[5]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[6] GENE FUSION WITH AN ETS DNA-BINDING DOMAIN CAUSED BY CHROMOSOME-TRANSLOCATION IN HUMAN TUMORS [J].

DELATTRE, O ;

ZUCMAN, J ;

PLOUGASTEL, B ;

DESMAZE, C ;

MELOT, T ;

PETER, M ;

KOVAR, H ;

JOUBERT, I ;

DEJONG, P ;

ROULEAU, G ;

AURIAS, A ;

THOMAS, G .

NATURE, 1992, 359 (6391) :162-165

[7] LOCATIONS OF THE ETS SUBFAMILY MEMBERS NET, ELK1, AND SAP1 (ELK3, ELK1, AND ELK4) ON 3 HOMOLOGOUS REGIONS OF THE MOUSE AND HUMAN GENOMES [J].

GIOVANE, A ;

SOBIESZCZUK, P ;

MIGNON, C ;

MATTEI, MG ;

WASYLYK, B .

GENOMICS, 1995, 29 (03) :769-772

[8] FUSION OF PDGF RECEPTOR-BETA TO A NOVEL ETS-LIKE GENE, TEL, IN CHRONIC MYELOMONOCYTIC LEUKEMIA WITH T(512) CHROMOSOMAL TRANSLOCATION [J].

GOLUB, TR ;

BARKER, GF ;

LOVETT, M ;

GILLILAND, DG .

CELL, 1994, 77 (02) :307-316

[9] FUSION OF THE TEL GENE ON 12P13 TO THE AML1 GENE ON 21Q22 IN ACUTE LYMPHOBLASTIC-LEUKEMIA [J].

GOLUB, TR ;

BARKER, GF ;

BOHLANDER, SK ;

HIEBERT, SW ;

WARD, DC ;

BRAYWARD, P ;

MORGAN, E ;

RAIMONDI, SC ;

ROWLEY, JD ;

GILLILAND, DG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (11) :4917-4921

[10]

ICHIKAWA H, 1994, CANCER RES, V54, P2865