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Differential effect of PKA on the Ca2+ release kinetics of the type I and IIIInSP3 receptors

被引：19
作者：
Dyer, JL
Mobasheri, H
Lea, EJA
Dawson, AP
Michelangeli, F [1 ]
机构：
[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England
[2] Univ E Anglia, Sch Biol Sci, Norwich NR4 7TJ, Norfolk, England
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 302卷 / 01期
基金：
英国惠康基金;
关键词：
InsP(3) receptors; Ca2+ channel; protein kinase A; phosphorylation; isoforms;
D O I：
10.1016/S0006-291X(03)00120-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of protein kinase A (PKA) on the inositol 1,4,5-trisphosphate (InSP3) receptor isoforms type I and type III were studied. The effects of PKA on the extent and rate constants for InSP3-induced Ca2+ release (IICR) were different for the two isoforms. The effects of PKA on the type I isoform showed a biphasic relationship dependent upon the concentration of PKA used. At low concentrations of PKA (<50 U/ml), both the extent and rate constants for IICR increased, while at higher concentrations (>200 U/ml) the extent and rate constants decreased. The type III isoform showed only an increase in the extent of IICR and not in the rate constants. The effects of PKA on the type I InSP3 receptor using single channel electrophysiological studies were also investigated. The stimulatory effect of PKA is due to an increase in conductance levels and not to a change in the mean open time of the channel. (C) 2003 Elsevier Science (USA). All rights reserved.
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收藏
页码：121 / 126
页数：6
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