Water distribution and related morphology in human stratum corneum at different hydration levels

被引:230
作者
Bouwstra, JA
de Graaff, A
Gooris, GS
Nijsse, J
Wiechers, JW
van Aelst, AC
机构
[1] Leiden Univ, Gorlaeus Labs, Leiden Amsterdam Ctr Drug Res, NL-2300 RA Leiden, Netherlands
[2] Uniqema, Gouda, Netherlands
[3] Univ Wageningen & Res Ctr, Dept Plant Physiol, Wageningen, Netherlands
[4] Univ Wageningen & Res Ctr, Dept Plant Cell Biol, Wageningen, Netherlands
关键词
swelling; water; stratum corneum; distribution;
D O I
10.1046/j.1523-1747.2003.12128.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
This study focused on the water distribution in human stratum corneum and on the swelling of the corneocytes. For this purpose stratum corneum was hydrated to various levels and used either for Fourier transform infrared spectroscopy or for cryo-scanning electron microscopy. The images were analyzed with respect to water localization and cell shape. The Fourier transform infrared spectra were measured to study the water-lipid interactions. The results show that water only slightly changes the lipid transitions in the stratum corneum even at a hydration level of 300% wt/wt compared to stratum corneum and that water is inhomogeneously distributed in the stratum corneum. No gradual increase in water level was observed in depth. At 57%-87% wt/wt water content the hydration level in the central part of stratum corneum is higher than in the superficial and deeper cell layers. Water domains are mainly present within the corneocytes and not in the intercellular regions. At a very high hydration level (300% wt/wt), the corneocytes are strongly swollen except for the deepest cell layers adjacent to the viable epidermis. The corneocytes in these layers are not swollen. At 300% wt/wt hydration level water domains are also present in intercellular regions. Between 17% wt/wt and 300% wt/wt the cell thickness increases linearly with the hydration level suggesting that swelling of cells mainly occurs in the direction perpendicular to the skin surface. At an increased hydration level, the corneocyte envelope more efficiently surrounds the cell content compensating for the increased cell volume. The changes in stratum corneum morphology with increasing water level have also been observed in dermatomed skin.
引用
收藏
页码:750 / 758
页数:9
相关论文
共 28 条
[1]   EFFECT OF HYDRATION UPON THE FLUIDITY OF INTERCELLULAR MEMBRANES OF STRATUM-CORNEUM - AN EPR STUDY [J].
ALONSO, A ;
MEIRELLES, NC ;
TABAK, M .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1237 (01) :6-15
[2]   THE DIFFUSION OF WATER ACROSS THE STRATUM-CORNEUM AS A FUNCTION OF ITS WATER-CONTENT [J].
BLANK, IH ;
MOLONEY, J ;
EMSLIE, AG ;
SIMON, I ;
APT, C .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1984, 82 (02) :188-194
[3]   EXAMINATION OF STRATUM-CORNEUM BARRIER FUNCTION INVIVO BY INFRARED-SPECTROSCOPY [J].
BOMMANNAN, D ;
POTTS, RO ;
GUY, RH .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1990, 95 (04) :403-408
[4]   STRUCTURE OF HUMAN STRATUM-CORNEUM AS A FUNCTION OF TEMPERATURE AND HYDRATION - A WIDE-ANGLE X-RAY-DIFFRACTION STUDY [J].
BOUWSTRA, JA ;
GOORIS, GS ;
SALOMONSDEVRIES, MA ;
VANDERSPEK, JA ;
BRAS, W .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1992, 84 (03) :205-216
[5]   STRUCTURAL INVESTIGATIONS OF HUMAN STRATUM-CORNEUM BY SMALL-ANGLE X-RAY-SCATTERING [J].
BOUWSTRA, JA ;
GOORIS, GS ;
VANDERSPEK, JA ;
BRAS, W .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (06) :1005-1012
[6]   USE OF DIFFERENTIAL THERMAL ANALYSIS TO STUDY BOUND WATER IN STRATUM CORNEUM MEMBRANES [J].
BULGIN, JJ ;
VINSON, LJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 136 (03) :551-&
[7]   In vivo confocal Raman microspectroscopy of the skin:: Noninvasive determination of molecular concentration profiles [J].
Caspers, PJ ;
Lucassen, GW ;
Carter, EA ;
Bruining, HA ;
Puppels, GJ .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 116 (03) :434-442
[8]   Modes of action of terpene penetration enhancers in human skin differential scanning calorimetry, small-angle X-ray diffraction and enhancer uptake studies [J].
Cornwell, PA ;
Barry, BW ;
Bouwstra, JA ;
Gooris, GS .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1996, 127 (01) :9-26
[9]   CHARACTERIZATION OF LOW-TEMPERATURE (IE, LESS-THAN-65-DEGREES-C) LIPID TRANSITIONS IN HUMAN STRATUM-CORNEUM [J].
GAY, CL ;
GUY, RH ;
GOLDEN, GM ;
MAK, VHW ;
FRANCOEUR, ML .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 103 (02) :233-239
[10]   THE EFFECTS OF PROTECTIVE OCCLUSIVE GLOVES ON STRATUM-CORNEUM BARRIER PROPERTIES [J].
GRAVES, CJ ;
EDWARDS, C ;
MARKS, R .
CONTACT DERMATITIS, 1995, 33 (03) :183-187