Incidence of chromosomal abnormalities from a morphologically normal cohort of embryos in poor-prognosis patients

被引:109
作者
Magli, MC [1 ]
Gianaroli, L [1 ]
Munne, S [1 ]
Ferraretti, AP [1 ]
机构
[1] SISMER, Reprod Med Unit, I-40138 Bologna, Italy
关键词
aneuploidy; multicolor fluorescence in situ hybridization; multiple in vitro fertilization failures; poor-prognosis patients; preimplantation diagnosis;
D O I
10.1023/A:1022596528036
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Purpose: Preimplantation genetic diagnosis of aneuploidy was performed on the embryos yielded by 70 poor-prognosis patients, with the aim of transferring those with a normal chromosomal complement, thus possibly increasing the chances of pregnancy. Methods: Multicolor fluorescence in situ hybridization (FISH) uas applied for the simultaneous detection of chromosomes X, Y, 13, 16, 18, and 21. Inclusion criteria were (1) a maternal age of 36 years or older (n = 33), (2) three or more previous in vitro fertilization cycles (n = 20), and (3) art altered karyotype (n = 17). Results: A total of 412 embryos underwent FISH, resulting in 234 (57%) that were chromosomally abnormal. Euploid embryos were available for transfer in 59 patients, generating 19 pregnancies (32%), with an implantation rate of 19.9%. Conclusions: High rates of chromosomally abnormal embryos in poor-prognosis patients can determine repeated in vitro fertilization failures when embryo selection is performed on the basis of morphological criteria alone. Hence, the FISH analysis could represent the prevailing approach for the identification of embryos possessing full potential for developing to term.
引用
收藏
页码:297 / 301
页数:5
相关论文
共 11 条
[1]   Relationship of timing of agonist administration in the cycle phase to the ovarian response to gonadotropins in the long down-regulation protocols for assisted reproductive technologies [J].
Ferraretti, AP ;
Magli, C ;
Feliciani, E ;
Montanaro, N ;
Gianaroli, L .
FERTILITY AND STERILITY, 1996, 65 (01) :114-121
[2]   Will preimplantation genetic diagnosis assist patients with a poor prognosis to achieve pregnancy? [J].
Gianaroli, L ;
Magli, MC ;
Munne, S ;
Fiorentino, A ;
Montanaro, N ;
Ferraretti, AP .
HUMAN REPRODUCTION, 1997, 12 (08) :1762-1767
[3]  
Gianaroli L, 1997, INT J ANDROL, V20, P31
[4]   Preimplantation genetic diagnosis increases the implantation rate in human in vitro fertilization by avoiding the transfer of chromosomally abnormal embryos [J].
Gianaroli, L ;
Magli, MC ;
Ferraretti, AP ;
Fiorentino, A ;
Garrisi, J ;
Munne, S .
FERTILITY AND STERILITY, 1997, 68 (06) :1128-1131
[5]   Detection of chromosomal abnormalities in human preimplantation embryos using FISH [J].
Harper, JC ;
Delhanty, JDA .
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1996, 13 (02) :137-139
[6]   Treatment-related chromosome abnormalities in human embryos [J].
Munne, S ;
Magli, C ;
Adler, A ;
Wright, G ;
deBoer, K ;
Mortimer, D ;
Tucker, M ;
Cohen, J ;
Gianaroli, L .
HUMAN REPRODUCTION, 1997, 12 (04) :780-784
[7]   Simultaneous enumeration of chromosomes 13, 18, 21, X, and Y in interphase cells for preimplantation genetic diagnosis of aneuploidy [J].
Munne, S ;
Weier, HUG .
CYTOGENETICS AND CELL GENETICS, 1996, 75 (04) :263-270
[8]   EMBRYO MORPHOLOGY, DEVELOPMENTAL RATES, AND MATERNAL AGE ARE CORRELATED WITH CHROMOSOME-ABNORMALITIES [J].
MUNNE, S ;
ALIKANI, M ;
TOMKIN, G ;
GRIFO, J ;
COHEN, J .
FERTILITY AND STERILITY, 1995, 64 (02) :382-391
[9]   OOCYTE MATURATION, FERTILIZATION AND EMBRYONIC GROWTH-INVITRO [J].
PLACHOT, M ;
MANDELBAUM, J .
BRITISH MEDICAL BULLETIN, 1990, 46 (03) :675-694
[10]   The sperm centriole: Its inheritance, replication and perpetuation in early human embryos [J].
Sathanathan, AH ;
Ratnam, SS ;
Ng, SC ;
Tarin, JJ ;
Gianaroli, L ;
Trounson, A .
HUMAN REPRODUCTION, 1996, 11 (02) :345-356