Occurrence and diagnosis of Tomato chlorosis virus in Portugal

被引：66

作者：

Louro, D

Accotto, GP

Vaira, AM

机构：

[1] CNR, Ist Fitovirol Applicata, I-10135 Turin, Italy

[2] Direccao Geral Proteccao Culturas Quinta Marques, P-2784505 Oeiras, Portugal

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2000年 / 106卷 / 06期

关键词：

Closterovirus; Crinivirus; ToCV; tomato; whiteflies;

D O I：

10.1023/A:1008738130592

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Tomato chlorosis virus (ToCV), a new whitefly-transmitted and phloem-limited Crinivirus infecting tomatoes in Europe, is reported for the first time in Portugal. Tomato plants with symptoms of interveinal chlorosis, collected during autumn 1998 and summer and autumn 1999 in Algarve, southern Portugal, were positive in RT-PCR assays using ToCV-specific primers. The amplified 439 bp fragment was sequenced and showed 99% homology with the ToCV sequence in the GenBank database. A digoxigenin-DNA probe was produced and tested in dot-blot with total RNAs extracted from tomato samples. Both the RT-PCR and dot-blot hybridisation procedures enabled rapid and reliable detection of ToCV from field samples.

引用

页码：589 / 592

页数：4

共 7 条

[1] Typing of tomato yellow leaf curl viruses in Europe [J].