Synthesis of neoglycoproteins containing O-methylated trisaccharides related to excretory/secretory antigens of Toxocara larvae

被引：17

作者：

Amer, H ^{[1
]}

Hofinger, A ^{[1
]}

Kosma, P ^{[1
]}

机构：

[1] Univ Agr Sci, Inst Chem, A-1190 Vienna, Austria

来源：

CARBOHYDRATE RESEARCH | 2003年 / 338卷 / 01期

关键词：

neoglycoprotein; Toxocara; antigen; O-glycan; fucose;

D O I：

10.1016/S0008-6215(02)00355-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The disaccharides allyl beta-D-galactopyranosyl-(1 --> 3)-2-acetamido-2-deoxy-beta- and alpha-D-galactopyranoside 10a and 10b and the trisaccharides allyl 2-O-methyl-alpha-L-fucopyranosyl-(1 --> 2)-beta-D-galactopyranosyl-(1--> 3)-2-acetamido-2-deoxy-beta- and alpha-D-galactopyranoside 18a and 18b have been prepared using stepwise assembly of the sugar units. The glycosidic linkages were formed employing the trichloroacetimidate procedure for the attachment of the galactopyranosyl residue and N-iodosuccinimide/triflic acid activation of an ethyl 1-thiofucopyranoside donor for fucosylation. Deprotection furnished the allyl glycosides which were converted into cysteamine-spacered ligands, activated with thiophosgene and subsequently linked to bovine serum albumin. The neoglycoproteins serve as immunoreagents to determine epitope specificities of monoclonal antibodies directed against highly immunogenic O-glycans located at the surface of Toxocara larvae. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：35 / 45

页数：11

共 31 条

[1] Synthesis of O-methylated disaccharides related to excretory/secretory antigens of Toxocara larvae [J].