Detection of Listeria monocytogenes using a commercial PCR kit and different DNA extraction methods

被引:48
作者
Amagliani, G.
Giammarini, C.
Omiccioli, E.
Brandi, G.
Magnani, M.
机构
[1] Univ Urbino, Ctr Biotecnol, I-61032 Fano, PU, Italy
[2] Univ Urbino, Ist Sci Tossicol Igienist & Ambientali, I-61029 Urbino, PU, Italy
[3] Diatheva Srl, I-61032 Fano, PU, Italy
[4] Ist Zooprofilatt Sperimentale Umbria & Marche, I-06126 Perugia, Italy
[5] Univ Urbino, Ist Chim Biol G Fornaini, I-60129 Urbino, PU, Italy
关键词
Listeria monocytogenes; PCR detection kit; magnetic DNA extraction;
D O I
10.1016/j.foodcont.2006.06.012
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The aim of our work was to evaluate a new commercial test kit for the detection of Listeria monocytogenes by PCR, using different DNA extraction methods. Food samples (pork sausage and "mozzarella" cheese) were spiked with known concentrations of L. monocylogenes and culture-enriched for 24 It. DNA extracted using three commercial kits and two standard methods, was amplified in species-specific PCR employing a L. monocytogenes PCR Detection Kit (Diatheva). The PCR-based method proved to be a reliable means of detecting the pathogen in food samples independently from the extraction procedure used, even for a contamination cell number of 1 cfa/g before culture enrichment. The molecular assay, showing perfect agreement with standard microbiological tests and a considerably shortened analysis time, provides a sensitive and rapid alternative for applications in the testing of foods for microbiological contamination, and highlights the potential of PCR technology in routine food control. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1137 / 1142
页数:6
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