Identification, sequence, and expression of Treponema denticola mcpA, a putative chemoreceptor gene

被引：6

作者：

Greene, SR ^{[1
]}

Stamm, LV ^{[1
]}

机构：

[1] Univ N Carolina, Sch Publ Hlth, Dept Epidemiol, Program Infect Dis, Chapel Hill, NC 27599 USA

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 157卷 / 02期

关键词：

methyl-accepting chemotaxis protein; MCP; spirochete; periodontal disease; Treponema denticola;

D O I：

10.1111/j.1574-6968.1997.tb12780.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleotide sequence of a methyl-accepting chemotaxis protein gene, mcpA, from Treponema denticola has been determined. The mcpA gene encodes a 729-amino acid protein whose deduced amino acid sequence has significant homology with several bacterial MCPs. T. denticola McpA contains two N-terminal transmembrane regions and two C-terminal putative methylation sequences that art separated by a highly conserved signaling domain. The organization of these structural features is characteristic of MCPs. The observed molecular mass of the in vitro synthesized McpA (76.0 kDa) correlates with the predicted molecular mass of the protein (80.1 kDa).

引用

页码：245 / 249

页数：5

共 20 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] NUCLEOTIDE-SEQUENCE OF DCRA, A DESULFOVIBRIO-VULGARIS HILDENBOROUGH CHEMORECEPTOR GENE, AND ITS EXPRESSION IN ESCHERICHIA-COLI [J].

DOLLA, A ;

FU, RD ;

BRUMLIK, MJ ;

VOORDOUW, G .

JOURNAL OF BACTERIOLOGY, 1992, 174 (06) :1726-1733

[3] ASSEMBLY OF AN MCP RECEPTOR, CHEW, AND KINASE CHEA COMPLEX IN THE BACTERIAL CHEMOTAXIS SIGNAL TRANSDUCTION PATHWAY [J].

GEGNER, JA ;

GRAHAM, DR ;

ROTH, AF ;

DAHLQUIST, FW .

CELL, 1992, 70 (06) :975-982

[4] Evidence for a methyl-accepting chemotaxis protein gene (mcp1) that encodes a putative sensory transducer in virulent Treponema pallidum [J].

Hagman, KE ;

Porcella, SF ;

Popova, TG ;

Norgard, MV .

INFECTION AND IMMUNITY, 1997, 65 (05) :1701-1709

[5]

HANLON DW, 1994, J BIOL CHEM, V269, P14038

[6] IDENTIFICATION OF TLPC, A NOVEL 62-KDA MCP-LIKE PROTEIN FROM BACILLUS-SUBTILIS [J].

HANLON, DW ;

ROSARIO, MML ;

ORDAL, GW ;

VENEMA, G ;

VANSINDEREN, D .

MICROBIOLOGY-SGM, 1994, 140 :1847-1854

[7]

KATARIOU S, 1983, J BACTERIOL, V156, P95

[8] THE DETECTION AND CLASSIFICATION OF MEMBRANE-SPANNING PROTEINS [J].

KLEIN, P ;

KANEHISA, M ;

DELISI, C .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 815 (03) :468-476

[9] IMPROVEMENTS IN PROTEIN SECONDARY STRUCTURE PREDICTION BY AN ENHANCED NEURAL NETWORK [J].

KNELLER, DG ;

COHEN, FE ;

LANGRIDGE, R .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 214 (01) :171-182

[10] SENSORY TRANSDUCERS OF ESCHERICHIA-COLI ARE COMPOSED OF DISCRETE STRUCTURAL AND FUNCTIONAL DOMAINS [J].

KRIKOS, A ;

MUTOH, N ;

BOYD, A ;

SIMON, MI .

CELL, 1983, 33 (02) :615-622

← 1 2 →