CE-LIF-MSn profiling of oligosaccharides in human milk and feces of breast-fed babies

被引:72
作者
Albrecht, Simone [1 ]
Schols, Henk A. [1 ]
van den Heuvel, Ellen G. H. M. [2 ]
Voragen, Alphons G. J. [1 ]
Gruppen, Harry [1 ]
机构
[1] Wageningen Univ, Food Chem Lab, NL-6703 HD Wageningen, Netherlands
[2] FrieslandCampina Res, Deventer, Netherlands
关键词
Baby feces; Gastrointestinal tract; Human milk oligosaccharides; On-/off-line CE-LIF-MS; CAPILLARY-ELECTROPHORESIS; MASS-SPECTROMETRY; SIALYLATED OLIGOSACCHARIDES; LIQUID-CHROMATOGRAPHY; PRETERM; LACTOSE; ELECTROCHROMATOGRAPHY; COMBINATION; GLYCANS; LEWIS;
D O I
10.1002/elps.200900646
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Mixtures of the complex human milk oligosaccharides (HMOs) are difficult to analyze and gastrointestinal bioconversion products of HMOs may complicate analysis even more Their analysis, therefore, requires the combination of a sensitive and high-resolution separation technique with a mass identification tool This study introduces for the first time the hyphenation of CE with an electrospray mass spectrometer, capable to perform multiple MS analysis (ESI-MSn) for the separation and characterization of HMOs in breast milk and feces of breast-fed babies LIF was used for on- and off-line detections. From the overall 47 peaks detected in off-line CE-LIF electropherograms, 21 peaks could be unambiguously and 11 peaks could be tentatively assigned The detailed structural characterization of a novel lacto-N-neo-tetraose isomer and a novel lacto-N-fucopentaose isomer was established in baby feces and pointed to gastrointestinal hydrolysis of higher-Mw HMOs. CE-LIF-ESI-MSn presents, therefore, a useful tool which contributes to an advanced understanding on the fate of individual HMOs during their gastrointestinal passage
引用
收藏
页码:1264 / 1273
页数:10
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