Transmission-blocking effects of sera from malaria-exposed individuals on Plasmodium falciparum isolates from gametocyte carriers

被引:29
作者
Drakeley, CJ
Mulder, L
Tchuinkam, T
Gupta, S
Sauerwein, R
Targett, GAT
机构
[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England
[2] ORSTOM, OCEAC, Malaria Dept, Yaounde, Cameroon
[3] Univ Nijmegen Hosp, Dept Med Microbiol, NL-6500 HB Nijmegen, Netherlands
[4] Univ Oxford, Dept Zool, Oxford OX1 3PS, England
关键词
Plasmodium falciparum; transmission; mosquito infection; transmission-blocking activity;
D O I
10.1017/S0031182098002601
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Sera from donors exposed to malaria were tested for their ability to block the transmission of isolates from Cameroonian Plasmodium falciparum gametocyte carriers. Sera were selected from amongst Cameroonian and Gambian donors who had positive antibody reactivity against the surface of activated gametes and against epitopes of Pfs 48/45 (a potential transmission-blocking vaccine candidate antigen). Aliquots of washed blood from gametocyte carriers were resuspended in test and control sera and fed to An, gambiae mosquitoes via a membrane feeder. Comparisons of the prevalence and intensity of infections in dissected mosquitoes showed variations in the ability of sera to block the transmission of the different isolates. Sera were identified that had little or no blocking effect on the transmission of isolates unless the isolate was poorly infectious. Some sera completely blocked the transmission of some isolates whilst having little or no effect on others. The observed variation in transmission-modulating activity may have implications for the development of a transmission-blocking vaccine.
引用
收藏
页码:417 / 423
页数:7
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