Adrenomedullin has mitogenic effects on human oral keratinocytes: involvement of cyclic AMP

被引:45
作者
Kapas, S
Brown, DW
Farthing, PM
HagiPavli, E
机构
[1] Receptor Biology Group, Oral Diseases Research Centre, St. Bartholomew's Roy. London S., London E1 2AD, Turner Street
基金
英国医学研究理事会;
关键词
adrenomedullin; oral keratinocyte; cyclic AMP; mitogenesis;
D O I
10.1016/S0014-5793(97)01384-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of the novel vasoactive regulatory peptide, adrenomedullin, on human oral keratinocytes was investigated, Adrenomedullin, acting via its specific receptor, stimulated a dose-dependent increase in DNA synthesis, and, in addition, stimulated further changes in the cell cycle resulting in the proliferation of keratinocytes, When cells were incubated in the presence of increasing concentrations of adrenomedullin, there was a rapid and dose-dependent rise in intracellular cyclic AMP levels, Stimulation of mitogenesis and cell proliferation in these cells mere mimicked by the cell permeable cAMP analogue, dibutyryl cAMP, Adrenomedullin-stimulated mitogenesis was attenuated by the adenylyl cyclase inhibitor SQ22,536, but was unaffected by inhibitors of PKC, tyrosine kinase or the CGRP receptor antagonist, CGRP(8-37), These data identify adrenomedullin as a new mitogenic regulatory peptide of keratinocytes acting via the cAMP cascade. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:287 / 290
页数:4
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