Host gene expression in response to Egyptian broomrape (Orobanche aegyptiaca)

被引:25
作者
Griffitts, AA [1 ]
Cramer, CL [1 ]
Westwood, JH [1 ]
机构
[1] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA
关键词
parasitic plant; isoprenoid pathway; phenylpropanoid pathway; PR protein; farnesyltransferase; genetic engineering;
D O I
10.1614/WS-03-155R
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Selective control of Egyptian broomrape is extremely difficult because the close association between host crop and parasite limits the use of most mechanical and herbicidal approaches. However, this host-parasite interaction can also form the basis of the simplest control strategy: parasite-resistant crops. Although much work has been conducted to identify and characterize mechanisms of parasite resistance, varieties with stable resistance are still unavailable for most affected crops. The development of resistant crops can be accelerated by genetic engineering to the extent that important aspects of the host-parasite interaction are understood. In this Study, we characterize a variety of gene promoter elements with respect to parasite induction and expression pattern. Studies were conducted using transgenic plants expressing fusions of the beta-glucuronidase reporter gene with promoter elements from several genes. Promoters from genes known to have increased expression in response to pathogen attack or wounding showed localized, induced expression after parasitism. These included phenylalanine ammonia lyase, chalcone synthase, sesquiterpene cyclase, and HMG1 (3-hydroxy-3-methylglutaryl CoA reductase). In contrast, the systemic acquired resistance-associated gene PR-1a was not induced by parasitism. Non-defense-related genes varied in response, with squalene synthase being repressed, whereas farnesyltransferase was highly expressed in the region of parasite attachment. These results demonstrate a range of expression, both in intensity and tissue specificity, in response to parasitism.
引用
收藏
页码:697 / 703
页数:7
相关论文
共 46 条
[1]   PECTIN METHYLESTERASE IN CALLI AND GERMINATING-SEEDS OF OROBANCHE-AEGYPTIACA [J].
BENHOD, G ;
LOSNER, D ;
JOEL, DM ;
MAYER, AM .
PHYTOCHEMISTRY, 1993, 32 (06) :1399-1402
[2]   BIOSYNTHESIS AND METABOLISM OF SESQUITERPENOID PHYTOALEXINS AND TRITERPENOIDS IN POTATO CELL-SUSPENSION CULTURES [J].
BRINDLE, PA ;
KUHN, PJ ;
THRELFALL, DR .
PHYTOCHEMISTRY, 1988, 27 (01) :133-150
[3]  
CHAPPELL J, 1995, PLANT PHYSIOL, V107, P1, DOI 10.1145/224057.224059
[4]   LIPID-DERIVED SIGNALS THAT DISCRIMINATE WOUND-RESPONSIVE AND PATHOGEN-RESPONSIVE ISOPRENOID PATHWAYS IN PLANTS - METHYL JASMONATE AND THE FUNGAL ELICITOR ARACHIDONIC-ACID INDUCE DIFFERENT 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE GENES AND ANTIMICROBIAL ISOPRENOIDS IN SOLANUM-TUBEROSUM L [J].
CHOI, D ;
BOSTOCK, RM ;
AVDIUSHKO, S ;
HILDEBRAND, DF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2329-2333
[5]   DIFFERENTIAL INDUCTION AND SUPPRESSION OF POTATO 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE GENES IN RESPONSE TO PHYTOPHTHORA-INFESTANS AND TO ITS ELICITOR ARACHIDONIC-ACID [J].
CHOI, D ;
WARD, BL ;
BOSTOCK, RM .
PLANT CELL, 1992, 4 (10) :1333-1344
[6]   GENOMIC SEQUENCING [J].
CHURCH, GM ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995
[7]  
CRAMER CL, 1993, J NEMATOL, V25, P507
[8]   SCREENING TECHNIQUES AND SOURCES OF RESISTANCE TO PARASITIC ANGIOSPERMS [J].
CUBERO, JI ;
PIETERSE, AH ;
KHALIL, SA ;
SAUERBORN, J .
EUPHYTICA, 1994, 73 (1-2) :51-58
[9]  
Daniel MJ., 1995, SEED DEV GERMINATION, P567
[10]  
DOERNER PW, 1990, BIO-TECHNOL, V8, P845, DOI 10.1038/nbt0990-845