Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses

被引：65

作者：

Mumford, RA ^{[1
]}

Seal, SE ^{[1
]}

机构：

[1] Univ Greenwich, Nat Resources Inst, Chatham ME4 4TB, Kent, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 1997年 / 69卷 / 1-2期

关键词：

yam mosaic virus; yam mild mosaic virus; Dioscorea viruses; potyviridae; plant viral diagnostics; reverse transcription; polymerase chain reaction;

D O I：

10.1016/S0166-0934(97)00141-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) based assay has been developed for the detection of yam-infecting potyviruses. Based upon the same format two distinct simple tests have been developed, which allow the reliable diagnosis of yam mosaic virus and the tentatively named yam mild mosaic virus. By using immunocapture and a single-buffer RT-PCR reaction, the test can be performed in a single tube. The tests described have been shown to exhibit a thousand-fold increase in detection sensitivity compared to existing ELISA assays. (C) 1997 Elsevier Science B.V.

引用

页码：73 / 79

页数：7

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