Isolation and functional characterization of a novel plastidic hexokinase from Nicotiana tabacum

被引：67

作者：

Giese, JO

Herbers, K

Hoffmann, M

Klösgen, RB

Sonnewald, U

机构：

[1] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany

[2] SunGene GmbH & Co KGaA, D-06466 Gatersleben, Germany

[3] Univ Halle Wittenberg, Inst Pflanzenphysiol, D-06120 Halle An Der Saale, Germany

来源：

FEBS LETTERS | 2005年 / 579卷 / 03期

关键词：

hexokinase; stroma; starch sheath; xylem parenchyma; guard cell; root tip;

D O I：

10.1016/j.febslet.2004.12.071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Due to their central role in sugar metabolism and signalling plant hexokinases have been studied in great detail, however, little is known about the spatial and temporal expression and the sub-cellular distribution of individual hexokinase isoforms. Based on in planta and in vitro studies the recently isolated tobacco hexokinase 2 (Hxk2) could be located in the chloroplast stroma and biochemically characterized. Hxk2 represents the first innerplastidic hexokinase described from higher plants. Promoter studies indicate that Hxk2 is mainly expressed in cells of the vascular starch sheath and xylem parenchyma, in guard cells and root tips. We propose a role for Hxk2 in starch and secondary metabolism in the mentioned tissues. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：827 / 831

页数：5

共 29 条

[1] REDUCTANT FOR GLUTAMATE SYNTHASE IS GENERATED BY THE OXIDATIVE PENTOSE-PHOSPHATE PATHWAY IN NONPHOTOSYNTHETIC ROOT PLASTIDS [J].