Community-level physiological profiling performed with an oxygen-sensitive fluorophore in a microtiter plate

被引:42
作者
Garland, JL
Roberts, MS
Levine, LH
Mills, AL
机构
[1] Dynamac Corp, Kennedy Space Ctr, FL 32899 USA
[2] Univ Virginia, Dept Environm Sci, Microbial Ecol Lab, Charlottesville, VA 22904 USA
关键词
D O I
10.1128/AEM.69.5.2994-2998.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Community-level physiological profiling based upon fluorometric detection of oxygen consumption was performed on hydroponic rhizosphere and salt marsh litter samples by using substrate levels as low as 50 ppm with incubation times between 5 and 24 h. The rate and extent of response were increased in samples acclimated to specific substrates and were reduced by limiting nitrogen availability in the wells.
引用
收藏
页码:2994 / 2998
页数:5
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