Rapid and direct method for extraction of RNA from soil

被引：25

作者：

Borneman, J

Triplett, EW

机构：

[1] Univ Wisconsin, Dept Agron, Brock Inst Environm Microbiol, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 1997年 / 29卷 / 11-12期

关键词：

D O I：

10.1016/S0038-0717(97)00084-9

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

A rapid method for direct extraction of RNA from soil was developed. It is based on cell lysis by a bead beater procedure that optimizes extraction efficiency and minimizes shearing of RNA. The RNA was extracted from three very different soils and was free of DNA contamination. The extracted RNA was sufficiently pure to permit amplification by RT-PCR. Intact rRNA molecules of both prokaryotic and eukaryotic origin were extracted by this method and were of sufficient yield to visualize these molecules on agarose gels. (C) 1997 Elsevier Science Ltd.

引用

页码：1621 / 1624

页数：4

共 19 条

[1] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION
AMANN, RI
LUDWIG, W
SCHLEIFER, KH
[J]. MICROBIOLOGICAL REVIEWS, 1995, 59 (01) : 143 - 169
[2] Molecular microbial diversity of an agricultural soil in Wisconsin
Borneman, J
Skroch, PW
OSullivan, KM
Palus, JA
Rumjanek, NG
Jansen, JL
Nienhuis, J
Triplett, EW
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (06) : 1935 - 1943
[3] A METHOD TO ISOLATE RNA FROM GRAM-POSITIVE BACTERIA AND MYCOBACTERIA
CHEUNG, AL
EBERHARDT, KJ
FISCHETTI, VA
[J]. ANALYTICAL BIOCHEMISTRY, 1994, 222 (02) : 511 - 514
[4] PHYLOGENETIC STAINS - RIBOSOMAL RNA-BASED PROBES FOR THE IDENTIFICATION OF SINGLE CELLS
DELONG, EF
WICKHAM, GS
PACE, NR
[J]. SCIENCE, 1989, 243 (4896) : 1360 - 1363
[5] RNA DNA RATIOS AND DNA CONCENTRATIONS AS INDICATORS OF GROWTH-RATE AND BIOMASS IN PLANKTONIC MARINE ORGANISMS
DORTCH, Q
ROBERTS, TL
CLAYTON, JR
AHMED, SI
[J]. MARINE ECOLOGY PROGRESS SERIES, 1983, 13 (01) : 61 - 71
[6] QUANTITATIVE RELATIONSHIP BETWEEN NAPHTHALENE CATABOLIC GENE-FREQUENCY AND EXPRESSION IN PREDICTING PAH DEGRADATION IN SOILS AT TOWN GAS MANUFACTURING SITES
FLEMING, JT
SANSEVERINO, J
SAYLER, GS
[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1993, 27 (06) : 1068 - 1074
[7] HARRIS D, 1994, BEYOND THE BIOMASS, P111
[8] ESTIMATING THE GROWTH-RATE OF SLOWLY GROWING MARINE-BACTERIA FROM RNA-CONTENT
KEMP, PF
LEE, S
LAROCHE, J
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (08) : 2594 - 2601
[9] COMPARISON OF NUCLEIC-ACID HYBRIDIZATION AND FLUOROMETRY FOR MEASUREMENT OF THE RELATIONSHIP BETWEEN RNA/DNA RATIO AND GROWTH-RATE IN A MARINE BACTERIUM
KERKHOF, L
WARD, BB
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (05) : 1303 - 1309
[10] LAME DJ, 1991, NUCLEIC ACID TECHNIQ, P115

← 1 2 →