Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues

被引：252

作者：

Dubey, JP ^{[1
]}

机构：

[1] USDA ARS, Beltsville Agr Res Ctr, Inst Livestock & Poultry Sci, Parasite Biol & Epidemiol Lab, Beltsville, MD 20705 USA

来源：

VETERINARY PARASITOLOGY | 1998年 / 74卷 / 01期

关键词：

Toxoplasma gondii; tissue cysts; digestion; pepsin; neutralization; sodium bicarbonate;

D O I：

10.1016/S0304-4017(97)00135-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Modification of the acid pepsin digestion procedure to isolate Toxoplasma gondii from animal muscular tissues is described. Muscles were incubated for 60 min instead of 90 min and acid was neutralized with 1.2% (w/v) sodium bicarbonate instead by repeated washings and centrifugations. Published by Elsevier Science B.V.

引用

页码：75 / 77

页数：3

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