DNA fragment analysis by an affordable multi pie-channel capillary electrophoresis system

被引：26

作者：

Liu, MS ^{[1
]}

Amirkhanian, VD ^{[1
]}

机构：

[1] BioCal Technol Inc, Orange, CA 92867 USA

来源：

ELECTROPHORESIS | 2003年 / 24卷 / 1-2期

关键词：

capillary electrophoresis; DNA fragment analysis; fluorescence detection; light-emitting diode;

D O I：

10.1002/elps.200390036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We are demonstrating a cost-effective multichannel capillary electrophoresis system for a high-efficiency double-stranded DNA (dsDNA) fragments analysis. This bench-type high-performance DNA analysis (HDA(TM)) system uses fluorescence-type detection with inexpensive solid-state light sources and nonmoving integrated emission collection micro-optics. DNA samples are analyzed simultaneously by using a multiple usage and disposable multicapillary cartridge, which contains integrated capillary channels, optical fibers and an integrated sieving gel reservoir. Using commercially available dsDNA size markers as indicators, the HDA(TM) system provides high resolving power in 7 min separations. The system can hold a total of 192 samples in two 96-well polymerase chain reaction (PCR) plates, which can be automatically analyzed within 2.5 h. This affordable system can be used in laboratories to replace slab gel electrophoresis for routine and high-throughput dsDNA analysis.

引用

页码：93 / 95

页数：3

共 6 条

[1] CAPILLARY GEL AFFINITY ELECTROPHORESIS OF DNA FRAGMENTS [J].