Rapid and efficient transformation of diploid Medicago truncatula and Medicago sativa ssp falcata lines improved in somatic embryogenesis

被引：129

作者：

Trinh, TH ^{[1
]}

Ratet, P ^{[1
]}

Kondorosi, E ^{[1
]}

Durand, P ^{[1
]}

Kamaté, K ^{[1
]}

Bauer, P ^{[1
]}

Kondorosi, A ^{[1
]}

机构：

[1] CNRS, Inst Sci Vegetales, UPR 40, F-91198 Gif Sur Yvette, France

来源：

PLANT CELL REPORTS | 1998年 / 17卷 / 05期

关键词：

M-truncatula; M-falcata; MsEnod12A; MsEnod12B; Srglb3;

D O I：

10.1007/s002990050405

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants was further improved by three successive in vitro regeneration cycles resulting in the line R108-1(c3). Agrobacterium tumefaciens-mediated transformation of leaf explants was carried out with promoter-gus constructs of two early nodulins (MsEnod12A and MsEnod12B) and one late nodulin (Srglb3). The transgenic plants thus produced on all ex plants within 3-4 months remained diploid and were fertile. This protocol appears to be the most efficient and fastest reported so far for leguminous plants.

引用

页码：345 / 355

页数：11

共 37 条

[1] IDENTIFICATION OF 2 ALFALFA EARLY NODULIN GENES WITH HOMOLOGY TO MEMBERS OF THE PEA ENOD12 GENE FAMILY [J].

ALLISON, LA ;

KISS, GB ;

BAUER, P ;

POIRET, M ;

PIERRE, M ;

SAVOURE, A ;

KONDOROSI, E ;

KONDOROSI, A .

PLANT MOLECULAR BIOLOGY, 1993, 21 (02) :375-380

[2]

Barker D. G., 1990, Plant Molecular Biology Reporter, V8, P40, DOI 10.1007/BF02668879

[3] Nod factors and cytokinins induce similar cortical cell division, amyloplast deposition and MsEnod12A expression patterns in alfalfa roots [J].

Bauer, P ;

Ratet, P ;

Crespi, MD ;

Schultze, M ;

Kondorosi, A .

PLANT JOURNAL, 1996, 10 (01) :91-105

[4] ALFALFA ENOD12 GENES ARE DIFFERENTIALLY REGULATED DURING NODULE DEVELOPMENT BY NOD FACTORS AND RHIZOBIUM INVASION [J].

BAUER, P ;

CRESPI, MD ;

SZECSI, J ;

ALLISON, LA ;

SCHULTZE, M ;

RATET, P ;

KONDOROSI, E ;

KONDOROSI, A .

PLANT PHYSIOLOGY, 1994, 105 (02) :585-592

[5]

BAUER P, 1997, MOL PLANT MICROBE IN, V10, P91

[6] NUCLEOTIDE-SEQUENCE AND EXACT LOCALIZATION OF THE NEOMYCIN PHOSPHOTRANSFERASE GENE FROM TRANSPOSON TN5 [J].

BECK, E ;

LUDWIG, G ;

AUERSWALD, EA ;

REISS, B ;

SCHALLER, H .

GENE, 1982, 19 (03) :327-336

[7] GENOME SIZE AND BASE COMPOSITION IN MEDICAGO-SATIVA AND MEDICAGO-TRUNCATULA SPECIES [J].

BLONDON, F ;

MARIE, D ;

BROWN, S ;

KONDOROSI, A .

GENOME, 1994, 37 (02) :264-270

[8] Transformation of barrel medic (Medicago truncatula Gaertn) by Agrobacterium tumefaciens and regeneration via somatic embryogenesis of transgenic plants with the MtENOD12 nodulin promoter fused to the gus reporter gene [J].

Chabaud, M ;

Larsonneau, C ;

Marmouget, C ;

Huguet, T .

PLANT CELL REPORTS, 1996, 15 (05) :305-310

[9] TRANSFORMATION OF MEDICAGO BY AGROBACTERIUM MEDIATED GENE-TRANSFER [J].

DEAK, M ;

KISS, GB ;

KONCZ, C ;

DUDITS, D .

PLANT CELL REPORTS, 1986, 5 (02) :97-100

[10]

Dellaporta S.L., 1983, Plant Molecular Biology Reporter, V1, P19, DOI DOI 10.1007/BF02712670

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