Ionizing Radiation Enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Induced Apoptosis through Up-Regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in Human Osteosarcoma Cells

被引：30

作者：

Hori, Takeshi ^{[1
,2
]}

Kondo, Takashi ^{[1
]}

Kanamori, Masahiko ^{[2
]}

Tabuchi, Yoshiaki ^{[3
]}

Ogawa, Ryohei ^{[1
]}

Zhao, Qing-Li ^{[1
]}

Ahmed, Kanwal ^{[1
]}

Yasuda, Taketoshi ^{[2
]}

Seki, Shoji ^{[2
]}

Suzuki, Kayo ^{[2
]}

Kimura, Tomoatsu ^{[2
]}

机构：

[1] Toyama Univ, Grad Sch Med & Pharmaceut Sci, Dept Radiol Sci, Toyama 9300194, Japan

[2] Toyama Univ, Grad Sch Med & Pharmaceut Sci, Dept Orthopaed Surg, Toyama 9300194, Japan

[3] Toyama Univ, Life Sci Res Ctr, Div Mol Genet Res, Toyama 9300194, Japan

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2010年 / 28卷 / 06期

关键词：

TRAIL; ionizing radiation; apoptosis; DR5; TRAIL; INHIBITION; INDUCTION; SARCOMA; MEMBER; LINES;

D O I：

10.1002/jor.21056

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL-induced Bid and caspase-3 activations. Decreases in the expression levels of the antiapoptotic proteins c-FLIP and MAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:739-745, 2010

引用

页码：739 / 745

页数：7

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