Inosine 5′-triphosphate can dramatically increase the yield of NASBA products targeting GC-rich and intramolecular base-paired viroid RNA

被引：8

作者：

Nakahara, K ^{[1
]}

Hataya, T ^{[1
]}

Uyeda, I ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Agr, Dept Agrobiol & Bioresources, Sapporo, Hokkaido 0608589, Japan

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 07期

关键词：

D O I：

10.1093/nar/26.7.1854

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleic acid sequence-based amplification (NASBA) according to the standard protocol failed to amplify cRNA of viroids, probably because of their CC-rich and intramolecular base-paired structure. However, NASBA in the presence of inosine 5'-triphosphate successfully amplified the cRNAs to viroids in total nucleic acid extracts from citrus plants. As sequence specificity of the cRNA to viroids was confirmed by northern analysis, the amplification and fidelity of cRNAs are sufficient for the sensitive and specific detection of viroids.

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页码：1854 / 1855

页数：2

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