Characterization of a rhamnogalacturonan and a xyloglucan from Nerium indicum and their activities on PC12 pheochromocytoma cells

被引:29
作者
Ding, K
Fang, JN [1 ]
Dong, TX
Tsim, KWK
Wu, HM
机构
[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
[2] Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China
[3] Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China
来源
JOURNAL OF NATURAL PRODUCTS | 2003年 / 66卷 / 01期
关键词
D O I
10.1021/np020118o
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Two polysaccharides, a rhamnogalacturonan (J1) and a xyloglucan (J2), were isolated and purified from the whole flowers of Nerium indicum and characterized by GC, GC-MS, NMR, and ESMS. The rhamnogalacturonan was found to consist of repeating units of a linear trisaccharide with the structure [-->-4)-alpha-GalpA(1-->2)alpha-Rhap(1-->4)-alpha-GalpA(1-->4)-](n), while the xyloglucan consisted of repeating units of the trisaccharide, which was (1-->4)-linked beta-glucopyranosyl residues as a backbone and substituted at position 6 linked to terminal alpha-xylopyranose side chains. These two polysaccharides were tested on the proliferation and differentiation on PC12 pheochromocytoma cells and found to have effects similar to nerve growth factor (NGF).
引用
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页码:7 / 10
页数:4
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