Interleukin-1β inhibits growth factor-stimulated restoration of wounded rat gastric epithelial cell monolayers

被引:20
作者
Nakamura, E [1 ]
Takahashi, S
Matsui, H
Okabe, S
机构
[1] Kyoto Pharmaceut Univ, Dept Appl Pharmacol, Kyoto 607, Japan
[2] Univ Tsukuba, Inst Clin Med, Inst Phys & Chem Sci, Cell Bank, Tsukuba, Ibaraki 305, Japan
关键词
interleukin-1; beta; gastric epithelial cell; growth factor; wound repair; RGM1;
D O I
10.1023/A:1018890419648
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
We examined the effect of interleukin-1 beta (IL-1 beta) on spontaneous and enhanced restoration (cell migration and proliferation) using an in vitro wound model comprising a confluent monolayer of rat gastric epithelial RGM1 cells. Repair of an artificial wound in a cell monolayer was found to be time-and concentration-dependent when the cells were incubated with epidermal growth factor (EGF) or transforming growth factor (TGF)-alpha alone for up to 24 hr. The growth factors also stimulated DNA synthesis significantly for 24 hr in a concentration-related manner: IL-1 beta had no effect on wound restoration in the absence of the growth factors. However, it markedly inhibited the restoration enhanced by EGF and TGF-alpha, the inhibition being about 60% and 704%;, respectively. In addition, IL-1 beta significantly reduced the DNA synthesis stimulated by the growth factors. The EGF-and TGF-alpha-enhanced restoration was reduced by about 30% by mitomycin C, which potently inhibited the stimulated DNA synthesis. Mitomycin C had no effect on the spontaneous restoration. Even when treated with mitomycin C, the inhibitory effect of IL-1 beta on the enhanced wound repair was still observed; however, the extent of the inhibition was decreased. These results indicate that IL-1 beta inhibits the migration as well as the proliferation of gastric epithelial cells enhanced by EGF and TGF-alpha, resulting in a failure of wound healing.
引用
收藏
页码:476 / 484
页数:9
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