Construction and characterization of an isogenic slt-ii deletion mutant of enterohemorrhagic Escherichia coli

被引：24

作者：

Gunzer, F

Bohn, U

Fuchs, S

Mühldorfer, I

Hacker, J

Tzipori, S

Donohue-Rolfe, A

机构：

[1] Tufts Univ, Sch Vet Med, Div Infect Dis, North Grafton, MA 01536 USA

[2] Univ Wurzburg, Inst Mol Infekt Biol, D-97070 Wurzburg, Germany

来源：

INFECTION AND IMMUNITY | 1998年 / 66卷 / 05期

关键词：

D O I：

10.1128/IAI.66.5.2337-2341.1998

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Enterohemorrhagic Escherichia coli (EHEC) produces Shiga-like toxins (SLT), potent protein synthesis inhibitors. To further dissect the role of SLT-II in the course of disease, we have constructed E. coli TUV86-2, an isogenic SLT-II-negative mutant of EHEC strain 86-24. The slt-ii gene was inactivated by suicide vector mutagenesis. We also isolated derivatives of strain 86-24 that were cured of the phage carrying the toxin genes.

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页码：2337 / 2341

页数：5

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