Comparative proteome analysis of Mycobacterium tuberculosis grown under aerobic and anaerobic conditions

被引:121
作者
Starck, J
Källenius, G
Marklund, BI
Andersson, DI
Åkerlund, T
机构
[1] Swedish Inst Infect Dis Control, Dept Bacteriol, S-17182 Solna, Sweden
[2] Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden
[3] Univ Kalmar, Dept Chem & Biomed Sci, SE-39182 Kalmar, Sweden
来源
MICROBIOLOGY-SGM | 2004年 / 150卷
关键词
D O I
10.1099/mic.0.27284-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Data are presented from two-dimensional (2-D) PAGE analysis of Mycobacterium tuberculosis strain Harlingen grown during aerobic and anaerobic culture, according to a modified Wayne dormancy model. M. tuberculosis cultures were grown to the transition point between exponential growth and stationary phase in the presence of oxygen (7 days) and then part of the cultures was shifted to anaerobic conditions for 16 days. Growth declined similarly during aerobic and anaerobic conditions, whereas the ATP consumption rapidly decreased in the anaerobic cultures. 2-D PAGE revealed 50 protein spots that were either unique to, or more abundant during, anaerobic conditions and 16 of these were identified by MALDI-TOF These proteins were the a-crystalline homologue (HspX), elongation factor Tu (Tuf), GroEL2, succinyl-CoA: 3-oxoacid-CoA transferase (ScoB), mycolic acid synthase (CmaA2), thioredoxin (TrxB2), beta-ketoacyl-ACP synthase (KasB), L-alanine dehydrogenase (Ald), Rv2005c, Rv2629, Rv0560c, Rv2185c and Rv3866. Some protein spots were found to be proteolytic fragments, e.g. HspX and GroEL2. These data suggest that M. tuberculosis induces expression of about 1 % of its genes in response to dormancy.
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页码:3821 / 3829
页数:9
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