Isolation and cross-species amplification of microsatellite loci from the fucoid seaweeds Fucus vesiculosus, F-serratus and Ascophyllum nodosum (Heterokontophyta, Fucaceae)

被引:63
作者
Engel, CR
Brawley, SH
Edwards, KJ
Serrao, E
机构
[1] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England
[2] Univ Maine, Sch Marine Sci, Orono, ME 04469 USA
[3] Univ Algarve, FCMA, Ctr Ciencias Mar, P-8005139 Faro, Portugal
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 02期
关键词
cross-species amplification; Fucaceae; microsatellites; seaweeds;
D O I
10.1046/j.1471-8286.2003.00390.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Fucaceae is a family of brown seaweeds that dominate and frequently co-occur on North Atlantic rocky shores. We developed nine polymorphic microsatellite markers for the fucoid seaweeds Fucus vesiculosus, F. serratus and Ascophyllum nodosum using a combined, enriched library. Six of these loci were polymorphic in at least two species, showing from two to eight alleles with heterozygosities ranging from 0.41 to 0.85. Loci were also tested on F. spiralis, revealing five polymorphic microsatellite loci in this species.
引用
收藏
页码:180 / 182
页数:3
相关论文
共 10 条
  • [1] CROSS-FERTILIZATION IN FUCOID SEAWEEDS
    BOLWELL, GP
    CALLOW, JA
    CALLOW, ME
    EVANS, LV
    [J]. NATURE, 1977, 268 (5621) : 626 - 627
  • [2] Characterization of microsatellite loci in the marine seaweeds, Fucus serratus and F-evanescens (Heterokontophyta; Fucaceae)
    Coyer, JA
    Veldsink, JH
    Jones, K
    Stam, WT
    Olsen, JL
    [J]. MOLECULAR ECOLOGY NOTES, 2002, 2 (01): : 35 - 37
  • [3] Edwards KJ, 1996, BIOTECHNIQUES, V20, P758
  • [4] Artificial hybridization between Ascophyllum nodosum and Fucus vesiculosus (Phaeophyceae) from Nova Scotia, Canada
    Kim, KY
    OLeary, SJ
    Garbary, DJ
    [J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1997, 75 (07): : 1133 - 1136
  • [5] KNIEP H, 1925, FLORA, V118, P331
  • [6] Olsen JL, 2002, MOL ECOL NOTES, V2, P33
  • [7] Pritchard JK, 2000, GENETICS, V155, P945
  • [8] Rozen S, 2000, Methods Mol Biol, V132, P365
  • [9] SCOTT GW, 1994, CRYPTOGAMIE ALGOL, V15, P297
  • [10] Evolution of the Fucaceae (Phaeophyceae) inferred from nrDNA-ITS
    Serrao, EA
    Alice, LA
    Brawley, SH
    [J]. JOURNAL OF PHYCOLOGY, 1999, 35 (02) : 382 - 394