Molecularly imprinted solid-phase extraction method for the high-performance liquid chromatographic analysis of fungicide pyrimethanil in wine

被引:84
作者
Baggiani, C. [1 ]
Baravalle, P. [1 ]
Giraudi, G. [1 ]
Tozzi, C. [1 ]
机构
[1] Univ Turin, Dept Analyt Chem, Lab Bioanalyt Chem, I-10125 Turin, Italy
关键词
molecular imprinting; solid-phase extraction; wine analysis; fungicide; pyrimethanil;
D O I
10.1016/j.chroma.2006.12.016
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for molecularly imprinted solid-phase extraction (MISPE) of the fungicide pyrimethanil from wine samples has been investigated. The molecular imprinted polymer was obtained by iniferter-mediated grafting on porous chloromethylated polystyrene beads, using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The imprinted beads were evaluated for use as a solid-phase extraction sorbent, in order to develop the extraction protocol in aqueous standards and red wine samples. The optimised extraction protocol resulted in a reliable MISPE method suitable for HPLC analysis (stationary phase: Cromolith Performance C 18 column, 100 mm x 4.6 mm; mobile phase: acetonitrile-water (3:2, v/v), flow-rate: 1.00 ml/min; detection 270 nm). It was selective for pyrimethanil and the related pyrimidinic fungicides cyprodinil and mepanipyrim, while the non-pyrimidinic fungicides benalaxyl, chlozolinate, furalaxyl, iprodione, metalaxyl, nuarimol, procymidone and vinclozolin were not extracted. Recoveries performed on a wine matrix spiked with pyrimethanil at three different concentration levels were reproducible and were in good agreement with the recoveries performed on buffer, coming out between 80 and 90% (85 +/- 7.0% at 0.50 mu g/ml, 79 +/- 1.6% at 2.0 mu g/mI and 87 +/- 5.6% at 20 mu g/ml). Preconcentration and quantitative extraction of pyrimethanil from wine samples was shown to be feasible down to 0.1 mu g/ml. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:158 / 164
页数:7
相关论文
共 26 条
  • [1] Molecular imprinting for drug bioanalysis - A review on the application of imprinted polymers to solid-phase extraction and binding assay
    Andersson, LI
    [J]. JOURNAL OF CHROMATOGRAPHY B, 2000, 739 (01): : 163 - 173
  • [2] Comparison of pyrimethanil-imprinted beads and bulk polymer as stationary phase by non-linear chromatography
    Baggiani, C
    Baravalle, P
    Anfossi, L
    Tozzi, C
    [J]. ANALYTICA CHIMICA ACTA, 2005, 542 (01) : 125 - 134
  • [3] Selectivity features of molecularly imprinted polymers recognising the carbamate group
    Baggiani, C
    Anfossi, L
    Baravalle, P
    Giovannoli, C
    Tozzi, C
    [J]. ANALYTICA CHIMICA ACTA, 2005, 531 (02) : 199 - 207
  • [4] Pesticide residues in grapes, wine, and their processing products
    Cabras, P
    Angioni, A
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (04) : 967 - 973
  • [5] Synthesis and application of an oxytetracycline imprinted polymer for the solid-phase extraction of tetracycline antibiotics
    Caro, E
    Marcé, RM
    Cormack, PAG
    Sherrington, DC
    Borrull, F
    [J]. ANALYTICA CHIMICA ACTA, 2005, 552 (1-2) : 81 - 86
  • [6] Immunoaffinity solid-phase extraction for the trace-analysis of low-molecular-mass analytes in complex sample matrices
    Delaunay, N
    Pichon, V
    Hennion, MC
    [J]. JOURNAL OF CHROMATOGRAPHY B, 2000, 745 (01): : 15 - 37
  • [7] GUZMANVAZQUEZ DA, 2006, J PHARM BIOMED ANAL, V40, P281
  • [8] Immuno-based sample preparation for trace analysis
    Hennion, MC
    Pichon, V
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2003, 1000 (1-2) : 29 - 52
  • [9] HENNION MC, 1994, ENVIRON SCI TECHNOL, V28, pA576, DOI 10.1021/es00062a717
  • [10] The analysis of beta-agonists in bovine muscle using molecular imprinted polymers with ion trap LCMS screening
    Kootstra, PR
    Kuijpers, CJPF
    Wubs, KL
    van Doorn, D
    Sterk, SS
    van Ginkel, LA
    Stephany, RW
    [J]. ANALYTICA CHIMICA ACTA, 2005, 529 (1-2) : 75 - 81