Screening for biomarkers in plasma from patients with gangrenous and phlegmonous appendicitis using CE and CEC in combination with MS

被引:20
作者
Johannesson, Nina
Olsson, Louise
Baeckstroem, Daniel
Wetterhall, Magnus
Danielsson, Rolf
Bergquist, Jonas
机构
[1] Uppsala Univ, Dept Phys & Analyt Chem, Biomed Ctr, S-75124 Uppsala, Sweden
[2] Karolinska Inst, Dept Med & Mol Surg, Stockholm, Sweden
关键词
appendicitis; biomarker; Fourier transform ion cyclotron resonance-MS; open tubular CEC; TOF-MS;
D O I
10.1002/elps.200600606
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Today a high degree of "false" appendicitis diagnoses are occurring. In this study, a screening experiment of biomarkers of two different kinds of appendicitis, gangrenous and phlegmonous, were conducted with CE and CEC coupled to MS. Plasma samples were obtained from patients pre- and post-surgery. A large amount of data was generated to be able to compare them, and chemometrics tools were utilized to visualize the differences. Indicative patterns were found for both pre- and post-surgery of the two types of inflammation as well as between them. The divergences were traced back to the MS peaks obtained in the CE- and CEC-MS setups as possible biomarkers for the two forms of appendicitis.
引用
收藏
页码:1435 / 1443
页数:9
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