A preliminary investigation of the application of differential scanning calorimetry to the study of collagen degradation in archaeological bone

被引:57
作者
Nielsen-Marsh, CM
Hedges, REM
Mann, T
Collins, MJ
机构
[1] Newcastle Univ, Postgrad Inst, NRG, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[2] Univ Oxford, Archaeol & Hist Art Res Lab, Oxford OX1 3QJ, England
[3] Perkin Elmer Thermal & Elemental Anal, Beaconsfield HP9 2GB, Bucks, England
基金
英国自然环境研究理事会;
关键词
DSC; archaeological bone; collagen; diagenesis;
D O I
10.1016/S0040-6031(00)00620-1
中图分类号
O414.1 [热力学];
学科分类号
摘要
The study investigated the potential application of differential scanning calorimetry (DSC) to archaeological bone collagen deterioration. The thermal transition of collagen was compared with the preservation state of the bones. Methods of sample preparation were shown to have a significant effect upon the ability to extract reproducible, reliable thermal data from the collagen. Three main protocols were examined, but the optimal method of collagen extraction (10%, w/v ethyldiamine tetraacetic acid demineralisation of bone shards) was slow, reducing the overall utility of DSC for archaeology. Comparison of the T-max (the maximum temperature of thermal transition) with the diagenetic state of the bone revealed no correlation with histological deterioration or alterations to the bone mineral or organic components. A correlation was observed, however, in young bone samples between T-max and age. This correlation was improved when thermal age, a parameter that integrates thermal history with the temperature dependence of collagen gelatinisation, was used. In thermally older bones T-max displayed little variation. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:129 / 139
页数:11
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