Identification of apigenin metabolites in rat liver perfusate by column switching high performance liquid chromatography/electrospray ionization mass spectrometry

被引：1

作者：

Basly, JP ^{[1
]}

Gradolatto, A

Teyssier, C

Canivenc-Lavier, NC

机构：

[1] UFR Pharm, UPRES EA Biomol & Cibles Cellulaires Tumorales 10, F-87025 Limoges, France

[2] INRA, UMR Toxicol Alimentaire 938, F-21065 Dijon, France

来源：

MONATSHEFTE FUR CHEMIE | 2003年 / 134卷 / 03期

关键词：

endocrine disrupters; apigenin; metabolites; HPLC; column switching; electrospray;

D O I：

10.1007/s00706-002-0553-0

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A selective column switching high performance liquid chromatographic method has been used for the simultaneous determination of apigenin and metabolites in rat liver perfusate. This new method clearly separated apigenin and the metabolites with high resolution. The structures of metabolites were proposed based on selective ion recording and full scan analysis by electrospray ionization mass spectrometry.

引用

页码：355 / 359

页数：5

共 12 条

[1] Extensive metabolism of the flavonoid chrysin by human Caco-2 and Hep G2 cells [J].

Galijatovic, A ;

Otake, Y ;

Walle, UK ;

Walle, T .

XENOBIOTICA, 1999, 29 (12) :1241-1256

[2] Metabolism of the anticancer drug flavopiridol, a new inhibitor of cyclin dependent kinases, in rat liver [J].

Jäger, W ;

Zembsch, B ;

Wolschann, P ;

Pittenauer, E ;

Senderowicz, AM ;

Sausville, EA ;

Sedlacek, HH ;

Graf, J ;

Thalhammer, T .

LIFE SCIENCES, 1998, 62 (20) :1861-1873

[3] Composition of flavonoids in fresh herbs and calculation of flavonoid intake by use of herbs in traditional Danish dishes [J].

Justesen, U ;

Knuthsen, P .

FOOD CHEMISTRY, 2001, 73 (02) :245-250

[4] Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β [J].

Kuiper, GGJM ;

Lemmen, JG ;

Carlsson, B ;

Corton, JC ;

Safe, SH ;

van der Saag, PT ;

van der Burg, P ;

Gustafsson, JÄ .

ENDOCRINOLOGY, 1998, 139 (10) :4252-4263

[5] Aromatase and 17β-hydroxysteroid dehydrogenase inhibition by flavonoids [J].

Le Bail, JC ;

Laroche, T ;

Marre-Fournier, F ;

Habrioux, G .

CANCER LETTERS, 1998, 133 (01) :101-106

[6] Quercetin is recovered in human plasma as conjugated derivatives which retain antioxidant properties [J].

Manach, C ;

Morand, C ;

Crespy, V ;

Demigné, C ;

Texier, O ;

Régérat, F ;

Rémésy, C .

FEBS LETTERS, 1998, 426 (03) :331-336

[7] Identification and quantification of flavonoids in human urine samples by column switching liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry [J].

Nielsen, SE ;

Freese, R ;

Cornett, C ;

Dragsted, LO .

ANALYTICAL CHEMISTRY, 2000, 72 (07) :1503-1509

[8] In vitro biotransformation of flavonoids by rat liver microsomes [J].

Nielsen, SE ;

Breinholt, V ;

Justesen, U ;

Cornett, C ;

Dragsted, LO .

XENOBIOTICA, 1998, 28 (04) :389-401

[9] Glucuronidation versus oxidation of the flavonoid galangin by human liver microsomes and hepatocytes [J].

Otake, Y ;

Hsieh, F ;

Walle, T .

DRUG METABOLISM AND DISPOSITION, 2002, 30 (05) :576-581

[10]

Teyssier C, 2000, DRUG METAB DISPOS, V28, P648

← 1 2 →