Use of oxyrase enzyme (Oxyrase®) for the detection of bacteriophages of Bacteroides fragilis in aerobic incubating conditions

被引：6

作者：

Puig, A ^{[1
]}

Jofre, J ^{[1
]}

Araujo, R ^{[1
]}

机构：

[1] Univ Barcelona, Dept Microbiol, E-08028 Barcelona, Spain

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1998年 / 31卷 / 03期

关键词：

Oxyrase (R); bacteriophages; Bacteroides fragilis; aerobic conditions;

D O I：

10.1016/S0167-7012(97)00103-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The biocatalytic oxygen reducing agent oxyrase enzyme (Oxyrase(R)) was shown to be useful for the detection of bacteriophages infecting the anaerobic bacteria Bacteroides fragilis, which have been proposed as potential indicators of human viruses and/or human faecal pollution in water. Oxyrase(R) provided very good conditions both for growing Bact. fragilis in liquid cultures in aerobic conditions and for the enumeration of bacteriophages by means of a triple agar layer for the detection of plaque-forming units. (C) 1998 Elsevier Science B.V.

引用

页码：205 / 207

页数：3

共 6 条

[1]

ADLER HI, 1983, BIOTECHNOL BIOENG, V13, P153

[2] SAFE SELF-CONTAINED CARBON DIOXIDE-HYDROGEN ANAEROBIC SYSTEM [J].

BREWER, JH ;

ALLGEIER, DL .

APPLIED MICROBIOLOGY, 1966, 14 (06) :985-&

[3] EVALUATION OF BACTEROIDES-FRAGILIS BACTERIOPHAGES AS INDICATORS OF THE VIROLOGICAL QUALITY OF WATER [J].

JOFRE, J ;

BOSCH, A ;

LUCENA, F ;

GIRONES, R ;

TARTERA, C .

WATER SCIENCE AND TECHNOLOGY, 1986, 18 (10) :167-173

[4]

LJUNGDAHL LG, 1986, MANUAL IND MICROBIOL

[5] BACTERIOPHAGES ACTIVE AGAINST BACTEROIDES-FRAGILIS IN SEWAGE-POLLUTED WATERS [J].

TARTERA, C ;

JOFRE, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (07) :1632-1637

[6] CULTURE AND DECONTAMINATION METHODS AFFECTING ENUMERATION OF PHAGES INFECTING BACTEROIDES-FRAGILIS IN SEWAGE [J].

TARTERA, C ;

ARAUJO, R ;

MICHEL, T ;

JOFRE, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (08) :2670-2673

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