Nucleic acid-triggered fluorescent probe activation by the staudinger reaction

被引:117
作者
Cai, JF [1 ]
Li, XX [1 ]
Yue, X [1 ]
Taylor, JS [1 ]
机构
[1] Washington Univ, Dept Chem, St Louis, MO 63130 USA
关键词
D O I
10.1021/ja0452626
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Highly sequence specific and sensitive detection systems for DNA or RNA in vivo would be of great use in biology and medicine for monitoring gene expression and diagnosing diseased cells. Herein we describe a new nucleic acid-triggered fluorescent probe system that is activated by a biocompatible and chemoselective Staudinger reaction. Copyright © 2004 American Chemical Society.
引用
收藏
页码:16324 / 16325
页数:2
相关论文
共 31 条
[1]   DETECTION OF NUCLEIC-ACID HYBRIDIZATION BY NONRADIATIVE FLUORESCENCE RESONANCE ENERGY-TRANSFER [J].
CARDULLO, RA ;
AGRAWAL, S ;
FLORES, C ;
ZAMECNIK, PC ;
WOLF, DE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8790-8794
[2]   STABILITY OF PEPTIDE NUCLEIC-ACIDS IN HUMAN SERUM AND CELLULAR-EXTRACTS [J].
DEMIDOV, VV ;
POTAMAN, VN ;
FRANKKAMENETSKII, MD ;
EGHOLM, M ;
BUCHARD, O ;
SONNICHSEN, SH ;
NIELSEN, PE .
BIOCHEMICAL PHARMACOLOGY, 1994, 48 (06) :1310-1313
[3]   Methods for visualizing RNA processing and transport pathways in living cells [J].
Dirks, RW ;
Molenaar, C ;
Tanke, HJ .
HISTOCHEMISTRY AND CELL BIOLOGY, 2001, 115 (01) :3-11
[4]   Study of the thermal decomposition of 2-azidoacetic acid by photoelectron and matrix isolation infrared spectroscopy [J].
Dyke, JM ;
Groves, AP ;
Morris, A ;
Ogden, JS ;
Dias, AA ;
Oliveira, AMS ;
Costa, ML ;
Barros, MT ;
Cabral, MH ;
Moutinho, AMC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (29) :6883-6887
[5]   PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES [J].
EGHOLM, M ;
BUCHARDT, O ;
CHRISTENSEN, L ;
BEHRENS, C ;
FREIER, SM ;
DRIVER, DA ;
BERG, RH ;
KIM, SK ;
NORDEN, B ;
NIELSEN, PE .
NATURE, 1993, 365 (6446) :566-568
[6]   Molecular beacons - Fluorogenic probes for living cell study [J].
Fang, XH ;
Mi, YM ;
Li, JWJ ;
Beck, T ;
Schuster, S ;
Tan, WH .
CELL BIOCHEMISTRY AND BIOPHYSICS, 2002, 37 (02) :71-81
[7]   RECENT ADVANCES IN THE STAUDINGER REACTION [J].
GOLOLOBOV, YG ;
KASUKHIN, LF .
TETRAHEDRON, 1992, 48 (08) :1353-1406
[8]   IL-2 or IL-4 mRNA as a potential flow cytometric marker molecule for selective collection of living T helper 1 or T helper 2 lymphocytes [J].
Ishibashi, K ;
Tsuji, A .
ANALYTICAL CHEMISTRY, 2003, 75 (11) :2715-2723
[9]   Detection and identification of single molecules in living cells using spectrally resolved fluorescence lifetime imaging microscopy [J].
Knemeyer, JP ;
Herten, DP ;
Sauer, M .
ANALYTICAL CHEMISTRY, 2003, 75 (09) :2147-2153
[10]   The Staudinger ligation -: A gift to chemical biology' [J].
Köhn, M ;
Breinbauer, R .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (24) :3106-3116