Recovery and regeneration of embryogenic cultures from female flowers of False Horn Plantain

被引:53
作者
Grapin, A
Ortíz, JL
Lescot, T
Ferrière, N
Côte, FX [1 ]
机构
[1] CATIE, Ctr Agron Trop Invest & Ensenanza, Unidad Biotecnol, Turrialba 7170, Costa Rica
[2] CIRAD, Ctr Cooperat Int Rech Agron Dev, F-34032 Montpellier 1, France
关键词
banana; histology; Musa AAB; plantain; somatic embryogenesis;
D O I
10.1023/A:1006423304033
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Somatic embryogenesis from immature male flowers in Musa is only suitable for genotypes with a male bud. Six friable embryogenic cultures were obtained from 28 cultured buds of female flowers of the AAB False Horn Plantains, 'Currare' and 'Currare Enano'. Embryogenic suspensions were established from these embryogenic cultures. Somatic embryogenesis was demonstrated histologicaly. Regeneration of plants was obtained either from somatic embryos directly isolated from embryogenic cultures or from suspensions after plating on a semi-solid medium. This study demonstrates that somatic embryogenesis from immature flowers is suitable for genotypes of Musa with or without male buds.
引用
收藏
页码:237 / 244
页数:8
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