The role of PopCel1 and PopCel2 in poplar leaf growth and cellulose biosynthesis

被引:35
作者
Ohmiya, Y
Nakai, T
Park, YW
Aoyama, T
Oka, A
Sakai, F
Hayashi, T [1 ]
机构
[1] Kyoto Univ, Wood Res Inst, Kyoto 6110011, Japan
[2] Kyoto Univ, Chem Res Inst, Kyoto 6110011, Japan
关键词
cellulase; cellulose biosynthesis; leaf growth; poplar;
D O I
10.1046/j.1365-313X.2003.01695.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Poplar calli transcribed two cellulase (endo-1,4-beta-glucanase) genes, PopCel1 and PopCel2 , whose mRNAs were differentially located in the growing leaves of poplar during cell wall synthesis. Histochemical and RT-PCR analyses of promoter-GUS fusion gene activities in transgenic poplar demonstrated that PopCel1 promoter-derived GUS activity was localized in the petiole and leaf veins, whereas PopCel2 was confined to mesophyll cells and disappeared from the tip during the development of leaves. Autoradiography of the leaf showed that the radioactivity of [C-14]sucrose incorporated into cellulose corresponded to the combination of the sucrose-induced tissue-specific patterns of PopCel1 and PopCel2 . Interestingly, 2,6-dichlorobenzonitrile (DCB) not only inhibited the incorporation of the radioactivity into cellulose, but also repressed the induction of both cellulase genes. Suppression of cellulases by expression of PopCel1 antisense cDNA or co-suppression of PopCel1 mRNA by overexpression of PopCel1 sense cDNA reduced leaf growth. Therefore, we came to the conclusion that PopCel1 and PopCel2 probably function to promote leaf growth in poplar by the endohydrolysis of 1,4-beta-glucan.
引用
收藏
页码:1087 / 1097
页数:11
相关论文
共 41 条
[1]   A MEMBRANE-ASSOCIATED FORM OF SUCROSE SYNTHASE AND ITS POTENTIAL ROLE IN SYNTHESIS OF CELLULOSE AND CALLOSE IN PLANTS [J].
AMOR, Y ;
HAIGLER, CH ;
JOHNSON, S ;
WAINSCOTT, M ;
DELMER, DP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9353-9357
[2]  
[Anonymous], [No title captured]
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   WEIGHT MATRIX DESCRIPTIONS OF 4 EUKARYOTIC RNA POLYMERASE-II PROMOTER ELEMENTS DERIVED FROM 502 UNRELATED PROMOTER SEQUENCES [J].
BUCHER, P .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 212 (04) :563-578
[5]   Auxin regulation and spatial localization of an endo-1,4-beta-D-glucanase and a xyloglucan endotransglycosylase in expanding tomato hypocotyls [J].
Catala, C ;
Rose, JKC ;
Bennett, AB .
PLANT JOURNAL, 1997, 12 (02) :417-426
[6]  
DEGRIERSON CJS, 1994, PLANT J, V5, P815
[7]   IDENTIFICATION OF A RECEPTOR PROTEIN IN COTTON FIBERS FOR THE HERBICIDE 2,6-DICHLOROBENZONITRILE [J].
DELMER, DP ;
READ, SM ;
COOPER, G .
PLANT PHYSIOLOGY, 1987, 84 (02) :415-420
[8]  
DuBois M., 1956, ANAL CHEM, V28, P50
[9]   Selection of marker-free transgenic plants using the isopentenyl transferase gene [J].
Ebinuma, H ;
Sugita, K ;
Matsunaga, E ;
Yamakado, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2117-2121
[10]   Differential ethylene-inducible expression of cellulase in pepper plants [J].
Ferrarese, L ;
Trainotti, L ;
Moretto, P ;
deLaureto, PP ;
Rascio, N ;
Casadoro, G .
PLANT MOLECULAR BIOLOGY, 1995, 29 (04) :735-747