Differential regulation of IL-8 by IL-1β and TNFα in hyaline membrane disease

被引:26
作者
Kwong, KY
Jones, CA
Cayabyab, R
Lecart, C
Stotts, CL
Randhawa, I
Ramanathan, R
Khuu, N
Minoo, P
机构
[1] Univ So Calif, Los Angeles Cty Med Ctr, Div Allergy & Immunol, Dept Pediat, Los Angeles, CA 90033 USA
[2] Univ So Calif, Los Angeles Cty Med Ctr, Dept Pediat, Med Ctr,Div Neonatol, Los Angeles, CA 90033 USA
[3] Univ So Calif, Los Angeles Cty Med Ctr, Dept Pediat, Womens & Childrens Hosp,Div Basic Res, Los Angeles, CA 90033 USA
关键词
interleukin (IL)-1 beta; tumor necrosis factor-alpha; IL-8; hyaline membrane disease; inflammation;
D O I
10.1023/A:1023244005765
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mechanisms that regulate cytokine-mediated inflammation in the lungs of preterm infants, including factors which regulate production of the chemokine IL-8, remain poorly defined. Sequential bronchoalveolar lavage samples were obtained from preterm newborns with hyaline membrane disease over a 28-day period. Bronchoalveolar lavage cell cytokine relationships were evaluated and the differential regulation of IL-8 by IL-1 beta and TNF alpha was studied in a short-term culture system. In vivo, IL-8 and IL-1 beta protein levels correlated closely with each other and with macrophage counts. In cell culture, exogenous anti-IL-1 beta antibody led to a 40% maximum inhibition (approximately) of IL-8 production by lipopolysaccharide stimulated lung inflammatory cells. Comparable amounts of exogenous anti-TNF alpha antibodies achieved a 15% maximum inhibition (approximately) of IL-8 production. Anti-IL-1 beta and anti-TNF alpha antibodies in combination did not inhibit IL-8 production beyond that achieved by anti-IL-1 beta antibody alone. These results, in preterm newborns, support the concept of lung inflammation mediated in part by a macrophage, IL-1 beta, and IL-8 cell cytokine pathway. The results also suggest that factors other than IL-1 beta and TNF alpha regulate IL-8 expression in the lungs of preterm infants.
引用
收藏
页码:71 / 80
页数:10
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