Chemisorbed poly(propylene sulphide)-based copolymers resist biomolecular interactions

被引:185
作者
Bearinger, JP [1 ]
Terrettaz, S
Michel, R
Tirelli, N
Vogel, H
Textor, M
Hubbell, JA
机构
[1] Univ Zurich, Inst Biomed Engn, CH-8044 Zurich, Switzerland
[2] Univ Zurich, Dept Mat Sci, CH-8044 Zurich, Switzerland
[3] Swiss Fed Inst Technol, Surface Sci & Technol Lab, CH-8044 Zurich, Schlieren, Switzerland
[4] Ecole Polytech Fed Lausanne, Inst Biomol Sci, CH-1015 Lausanne, Switzerland
关键词
D O I
10.1038/nmat851
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Alkanethiolates have been widely used as chemisorbates to modify gold surfaces, in spite of their relatively poor oxidative stability. We introduce gold-chemisorbing block copolymers bearing an anchoring block of poly(propylene sulphide) (PPS), selected in the expectation of greater stability. These materials offer a more robust approach to surface modification of gold. As an example, a triblock copolymer with poly(ethylene glycol) (PEG) was selected, with the goal of minimizing biological adsorption and adhesion. The copolymer PEG(17)-bl-PPS25-bl-PEG(g) chemisorbed to form a dense monolayer of 226 +/- 26 ng cm(-2), similar to2.2 nm thick. The copolymeric adlayer was much more stable to oxidation than commonly used alkanethiolates. Its presence greatly reduced protein adsorption (>95%), even after exposure to whole blood serum (>55 mg ml(-1)), as well as cell adhesion over long culture durations (>97%). PPS-containing copolymers are an attractive alternative to alkanethiolates, and PEG-bl-PPS-bl-PEG presents a powerful example for use in biodiagnostic and bioanalytical devices.
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收藏
页码:259 / 264
页数:6
相关论文
共 37 条
[1]   MANIPULATION OF THE WETTABILITY OF SURFACES ON THE 0.1-MICROMETER TO 1-MICROMETER SCALE THROUGH MICROMACHINING AND MOLECULAR SELF-ASSEMBLY [J].
ABBOTT, NL ;
FOLKERS, JP ;
WHITESIDES, GM .
SCIENCE, 1992, 257 (5075) :1380-1382
[2]  
ABUCHOWSKI A, 1977, J BIOL CHEM, V252, P3582
[3]   PREVENTION OF PROTEIN ADSORPTION AND PLATELET-ADHESION ON SURFACES BY PEO PPO PEO TRIBLOCK COPOLYMERS [J].
AMIJI, M ;
PARK, K .
BIOMATERIALS, 1992, 13 (10) :682-692
[4]   FORMATION OF MONOLAYER FILMS BY THE SPONTANEOUS ASSEMBLY OF ORGANIC THIOLS FROM SOLUTION ONTO GOLD [J].
BAIN, CD ;
TROUGHTON, EB ;
TAO, YT ;
EVALL, J ;
WHITESIDES, GM ;
NUZZO, RG .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (01) :321-335
[5]   Protein adsorption to oligo(ethylene glycol) self-assembled monolayers: Experiments with fibrinogen, heparinized plasma, and serum [J].
Benesch, J ;
Svedhem, S ;
Svensson, SCT ;
Valiokas, R ;
Liedberg, B ;
Tengvall, P .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2001, 12 (06) :581-597
[6]   Micropatterned immobilization of a G protein-coupled receptor and direct detection of G protein activation [J].
Bieri, C ;
Ernst, OP ;
Heyse, S ;
Hofmann, KP ;
Vogel, H .
NATURE BIOTECHNOLOGY, 1999, 17 (11) :1105-1108
[7]   Self-assembled monolayers: Recent developments and applications [J].
Bishop, AR ;
Nuzzo, RG .
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 1996, 1 (01) :127-136
[8]   X-ray photoelectron spectroscopy sulfur 2p study of organic thiol and disulfide binding interactions with gold surfaces [J].
Castner, DG ;
Hinds, K ;
Grainger, DW .
LANGMUIR, 1996, 12 (21) :5083-5086
[9]   MOLECULAR ORDER AT THE SURFACE OF AN ORGANIC MONOLAYER STUDIED BY LOW-ENERGY HELIUM DIFFRACTION [J].
CHIDSEY, CED ;
LIU, GY ;
ROWNTREE, P ;
SCOLES, G .
JOURNAL OF CHEMICAL PHYSICS, 1989, 91 (07) :4421-4423
[10]   Static secondary ion mass spectrometry studies of self-assembled monolayers: Influence of adsorbate chain length and terminal functional group on rates of photooxidation of alkanethiols on gold [J].
Cooper, E ;
Leggett, GJ .
LANGMUIR, 1998, 14 (17) :4795-4801