Electron and carbon balances in microbial fuel cells reveal temporary bacterial storage behavior during electricity generation

被引:205
作者
Freguia, Stefano [1 ]
Rabaey, Korneel [1 ]
Yuan, Zhiguo [1 ]
Keller, Jurg [1 ]
机构
[1] Univ Queensland, Adv Wastewater Management Ctr, Brisbane, Qld 4072, Australia
关键词
D O I
10.1021/es062611i
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Microbial fuel cells (MFCs) are emerging as a novel technology with a great potential to reduce the costs of wastewater treatment. Their most studied application is organic carbon removal. One of the parameters commonly used to quantify the performance of these cells is the Coulombic efficiency, i.e.,the electron recovery as electricity from the removed substrate. However, the "inefficiencies" of the process have never been fully identified. This study presents a method that uses the combination of electrochemical monitoring, chemical analysis, and a titration and off-gas analysis (TOGA) sensor to identify and quantify the sources of electron loss. The method was used successfully to close electron, carbon, and proton balances in acetate and glucose fed microbial fuel cells. The method revealed that in the case that a substrate is loaded as pulses carbon is stored inside the cells during initial high substrate conditions and consumed during starvation, with up to 57% of the current being generated after depletion of the external carbon source. Nile blue staining of biomass samples revealed lipophilic inclusions during high substrate conditions, thus confirming the storage of polymeric material in the bacterial cells. The method also allows for indirect measurement of growth yields, which ranged from 0 to 0.54 g biomass-C formed per g substrate-C used, depending on the type of substrate and the external resistance of the circuit.
引用
收藏
页码:2915 / 2921
页数:7
相关论文
共 16 条
[1]   Electrode-reducing microorganisms that harvest energy from marine sediments [J].
Bond, DR ;
Holmes, DE ;
Tender, LM ;
Lovley, DR .
SCIENCE, 2002, 295 (5554) :483-485
[2]   Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells [J].
Chaudhuri, SK ;
Lovley, DR .
NATURE BIOTECHNOLOGY, 2003, 21 (10) :1229-1232
[3]   Online titrimetric and off-gas analysis for examining nitrification processes in wastewater treatment [J].
Gapes, D ;
Pratt, S ;
Yuan, ZG ;
Keller, J .
WATER RESEARCH, 2003, 37 (11) :2678-2690
[4]  
Greenberg A. E., 1992, STANDARD METHODS EXA
[5]  
Heijnen J.J., 1999, ENCY BIOPROCESS TECH, P267
[6]   Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane [J].
Liu, H ;
Logan, BE .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2004, 38 (14) :4040-4046
[7]   Microbial fuel cells: Methodology and technology [J].
Logan, Bruce E. ;
Hamelers, Bert ;
Rozendal, Rene A. ;
Schrorder, Uwe ;
Keller, Jurg ;
Freguia, Stefano ;
Aelterman, Peter ;
Verstraete, Willy ;
Rabaey, Korneel .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2006, 40 (17) :5181-5192
[8]   Proton exchange membrane and electrode surface areas as factors that affect power generation in microbial fuel cells [J].
Oh, SE ;
Logan, BE .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 70 (02) :162-169
[9]   Impact of electrode composition on electricity generation in a single-compartment fuel cell using Shewanella putrefaciens [J].
Park, DH ;
Zeikus, JG .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 59 (01) :58-61
[10]   Development of a novel titration and off-gas analysis (TOGA) sensor for study of biological processes in wastewater treatment systems [J].
Pratt, S ;
Yuan, ZG ;
Gapes, D ;
Dorigo, M ;
Zeng, RJ ;
Keller, J .
BIOTECHNOLOGY AND BIOENGINEERING, 2003, 81 (04) :482-495